Ciliary Neurotrophic Factor Recombinant Protein | CNTF recombinant protein

Human Ciliary Neurotrophic Factor Recombinant

Cat. Num. AAA553037

• Purity: >98%, as determined by SDS-PAGE and HPLC
• Synonyms: Ciliary Neurotrophic Factor; Human Ciliary Neurotrophic Factor Recombinant; Ciliary Neurotrophic Factor ; cntf; CNTF recombinant protein

• Host: E Coli
• Purity/Purification: >98%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant Ciliary Neurotrophic Factor was lyophilized from a 0.2 um filtered PBS pH 7.5.
• Sequence: MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM
• Sequence Length: 200

• Domain: four helix cytokine core
• Host Note: Optimized DNA sequence encoding Human Ciliary Neurotrophic Factor mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human CNTF has a calculated molecular mass of approximately 22 kDa. Recombinant CNTF is a disulfide-linked monomer protein consisting of 200 amino acid residue subunits, and migrates as an approximately 22 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for CNTF recombinant protein

CNTF is found predominantly in peripheral nerve tissues. The main source appears to be myelin-associated Schwann cells in peripheral nerves and astrocytes in the central nervous system. CNTF is present at very high concentrations within intraocular tissues that contain the same muscle cells innervated by ciliary ganglionic neurons in vivo. CNTF receptors are expressed exclusively in the nervous system and skeletal muscle. The alpha subunit of the receptor is anchored to the cell membrane by a glycosyl- phosphatidylinositol linkage. The other receptor subunit, known as gp130, is also a component of receptors for LIF, Oncostatin M, IL6 and IL11, and seems to be involved also in signaling for CT-1. CNTF induces the expression of genes encoding hepatic acute phase proteins such as Haptoglobin, Alpha-1- antichymotrypsin, Alpha-2- Macroglobulin and Beta- fibrinogen in human hepatoma cells (HepG2) and in primary rat hepatocytes with a time course and dose-response comparable with that of IL6.

Product Categories/Family for CNTF recombinant protein

Cytokines

References

• the CNTF 1357 g >a polymorphism and the muscle strength response to resistance training. .J Appl Physiol, Jul 2009; 10.1152/japplphysiol.90835.2008.
• humanin inhibits neuronal cell death by interacting with a cytokine receptor complex or complexes involving CNTF receptor /wsx-1/gp130 .Mol. Biol. Cell, Jun 2009; 20: 2864 - 2873.
• effect of CNTF on retinal ganglion cell survival in experimental glaucoma .Invest. Ophthalmol. Vis. Sci., May 2009; 50: 2194 - 2200.
• CNTF over-expression leads to increased retinal ganglion cell survival in experimental glaucoma .Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 4052.
• CNTF induces rapid expression of neuronal and glial transcripts in cultured porcine retinal progenitor cells .Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 1266.