CNTF active protein

CNTF, 1-200aa, Human, His-tag, E Coli (Bioactivity Validated)

Cat. Num. AAA206462

• Gene Names: CNTF; HCNTF
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: CNTF; 1-200aa; Human; His-tag; E Coli (Bioactivity Validated); Ciliary neurotrophic factor; CNTF active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM
• Sequence Length: 200

• Applicable Applications for CNTF active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 for this effects is less or equal to 1 ug/ml.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CNTF active protein

CNTF (Ciliary neurotrophic factor) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. This protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF protein is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). It is localized in the cell nucleus subsequent to receptor binding. Recombinant human CNTF, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for CNTF active protein

Neuroscience

References

Sendther M., et al. (1994), J Neurobiol. 25 (11):1436-53.; Robert F., et al. (2007), J. Biol. Chem. 282 (46): 33421-33434

NCBI and Uniprot Product Information

• NCBI GI #: 4758020
• NCBI GeneID: 1270
• NCBI Accession #: NP_000605
• NCBI GenBank Nucleotide #: NM_000614.3
• UniProt Accession #: P26441; B2RAB2
• Molecular Weight: 25kDa (220aa), confirmed by MALDI-TOF
• NCBI Official Full Name: ciliary neurotrophic factor
• NCBI Official Synonym Full Names: ciliary neurotrophic factor
• NCBI Official Symbol: CNTF
• NCBI Official Synonym Symbols: HCNTF
• NCBI Protein Information: ciliary neurotrophic factor
• UniProt Protein Name: Ciliary neurotrophic factor
• Protein Family: Ciliary neurotrophic factor
• UniProt Gene Name: CNTF
• UniProt Synonym Gene Names: CNTF

NCBI Description

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010]

Uniprot Description

CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.