Ciliary Neurotrophic Factor Recombinant Protein | CNTF recombinant protein

Recombinant Ciliary Neurotrophic Factor (CNTF)

Cat. Num. AAA2029057

• Gene Names: CNTF; HCNTF
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Ciliary Neurotrophic Factor; Recombinant Ciliary Neurotrophic Factor (CNTF); CNTF recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MAFMEHSALT PHRRELCSRT IWLARKIRSD LTALTESYVK HQGLNKNINL DSVDGVPMAS TDQWSELTEA ERLQENLQAY RTFHIMLARL LEDQQVHFTP AEGDFHQAIH TLLLQVAAFA YQIEELMVLL ECNIPPKDAD GTPVIGGDGL FEKKLWGLKV LQELSHWTVR SIHDLRVISCHQTGIPAHGS HYIANDKEM
• Sequence Length: 200

• Applicable Applications for CNTF recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 26.4kDa
• Accurate Molecular Mass (KD): 27kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, T7 tag
• Organism Species: Oryctolagus cuniculus (Rabbit)
• Fragment: Met1~Met199
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4758020
• NCBI GeneID: 1270
• NCBI Accession #: NP_000605.1
• NCBI GenBank Nucleotide #: NM_000614.3
• Molecular Weight: 22,931 Da
• NCBI Official Full Name: ciliary neurotrophic factor
• NCBI Official Synonym Full Names: ciliary neurotrophic factor
• NCBI Official Symbol: CNTF
• NCBI Official Synonym Symbols: HCNTF
• NCBI Protein Information: ciliary neurotrophic factor
• UniProt Protein Name: Ciliary neurotrophic factor
• Protein Family: Ciliary neurotrophic factor
• UniProt Gene Name: CNTF
• UniProt Synonym Gene Names: CNTF
• UniProt Entry Name: CNTF_HUMAN

NCBI Description

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010]

Uniprot Description

CNTF: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. Belongs to the CNTF family.

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: extracellular space; cytoplasm

Molecular Function: interleukin-6 receptor binding; growth factor activity; ciliary neurotrophic factor receptor binding

Biological Process: negative regulation of photoreceptor cell differentiation; positive regulation of cell proliferation; regulation of retinal cell programmed cell death; neuron development; negative regulation of neuron apoptosis; signal transduction; muscle morphogenesis; growth; positive regulation of tyrosine phosphorylation of Stat3 protein