Western Blot (WB)
(Western BlotPositive WB detected in: SH-SY5Y whole cell lysate, U87 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysateAll lanes: YY1AP1 antibody at 4.3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 88, 84, 82, 81, 76, 80, 51, 96, 98 kDaObserved band size: 88, 51 kDa)
Rabbit anti-Human YY1AP1 Polyclonal Antibody | anti-YY1AP1 antibody
YY1AP1 Antibody
Protein G Purified
Protein G Purified
IHC: 1:200-1:500
IF: 1:50-1:200
Western Blot (WB)
(Western BlotPositive WB detected in: SH-SY5Y whole cell lysate, U87 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysateAll lanes: YY1AP1 antibody at 4.3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 88, 84, 82, 81, 76, 80, 51, 96, 98 kDaObserved band size: 88, 51 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in: SH-SY5Y whole cell lysate, U87 whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysateAll lanes: YY1AP1 antibody at 4.3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 88, 84, 82, 81, 76, 80, 51, 96, 98 kDaObserved band size: 88, 51 kDa)
Immunohistochemistry (IHC)
(IHC image of CSB-PA884488LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(IHC image of CSB-PA884488LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunofluorescence (IF)
(Immunofluorescence staining of HepG2 cells with CSB-PA884488LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of HepG2 cells with CSB-PA884488LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Testing Data
(Western BlotPositive WB detected in: SH-SY5Y whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, HL60 whole cell lysate, 293 whole cell lysateAll lanes: YY1AP1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 88, 84, 82, 81, 76, 80, 51, 96, 98 kDaObserved band size: 88 kDa)
Testing Data
(Western BlotPositive WB detected in: SH-SY5Y whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, HL60 whole cell lysate, 293 whole cell lysateAll lanes: YY1AP1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 88, 84, 82, 81, 76, 80, 51, 96, 98 kDaObserved band size: 88 kDa)
NCBI and Uniprot Product Information
NCBI Description
The encoded gene product presumably interacts with YY1 protein; however, its exact function is not known. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
Associates with the INO80 chromatin remodeling complex, which is responsible for transcriptional regulation, DNA repair, and replication (PubMed:27939641). Enhances transcription activation by YY1 (PubMed:14744866). Plays a role in cell cycle regulation (PubMed:17541814, PubMed:27939641).
Research Articles on YY1AP1
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Product Notes
The YY1AP1 yy1ap1 (Catalog #AAA7114646) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The YY1AP1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's YY1AP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:1000-1:5000 IHC: 1:200-1:500 IF: 1:50-1:200. Researchers should empirically determine the suitability of the YY1AP1 yy1ap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "YY1AP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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