Rabbit matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) ELISA Kit | MMP9 elisa kit

rabbit matrix metalloproteinase 9, MMP-9 ELISA Kit

Cat. Num. AAA703547

• Reactivity: Rabbit
• Synonyms: matrix metallopeptidase 9 (gelatinase B; 92kDa gelatinase; 92kDa type IV collagenase); rabbit matrix metalloproteinase 9; MMP-9 ELISA Kit; CLG4B; GELB; MANDP2; MMP-9; macrophage gelatinase; matrix metalloproteinase 9; matrix metalloproteinase 9 (gelatinase B; 92kDa type IV collagenase) ; type V collagenase; MMP9 elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rabbit MMP9. No significant cross-reactivity or interference between Rabbit MMP9 and analogues was observed.
• Sequence Length: 707

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Detection Range: 23.5 pg/ml-1500 pg/ml
• Sensitivity: 5.8 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Developmental Protein
• Protein Biological Process 3: Collagen degradation
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP9 elisa kit

Introduction: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Thrombospondins, intervertebral disc proteins, regulate the effective levels of matrix metalloproteinases (MMPs) 2 and 9, which are key effectors of ECM remodeling.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP-9. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated monoclonal antibody preparation specific for MMP-9 and incubated. Then substrate solution A and B are added to each well. Only those wells that contain MMP-9, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MMP-9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 126723483
• NCBI GeneID: 100008993
• NCBI Accession #: NP_001075672.1
• NCBI GenBank Nucleotide #: NM_001082203.1
• UniProt Accession #: P41246
• Molecular Weight: 78,308 Da
• NCBI Official Full Name: matrix metalloproteinase-9
• NCBI Official Symbol: MMP9
• NCBI Protein Information: matrix metalloproteinase-9; GELB; MMP-9; gelatinase B; 92 kDa gelatinase; 92 kDa type IV collagenase; matrix metalloproteinase 9
• UniProt Protein Name: Matrix metalloproteinase-9
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: MMP9
• UniProt Synonym Gene Names: MMP-9; GELB
• UniProt Entry Name: MMP9_RABIT

Uniprot Description

Function: Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments

Catalytic activity: Cleavage of gelatin types I and V and collagen types IV and V.

Cofactor: Binds 2 zinc ions per subunit