Human coagulation factor III (thromboplastin, tissue factor) ELISA Kit | TF elisa kit

Human tissue factor, TF ELISA Kit

Cat. Num. AAA702904

• Gene Names: F3; TF; TFA; CD142
• Reactivity: Human
• Synonyms: coagulation factor III (thromboplastin; tissue factor); Human tissue factor; TF ELISA Kit; Human tissue factor (TF) ELISA kit; CD142; TF; TFA; coagulation factor III; tissue factor; TF elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human TF. No significant cross-reactivity or interference between Human TF and analogues was observed.
• Sequence Length: 238

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 3.12 pg/ml -200 pg/ml
• Sensitivity: 0.78 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Blood Coagulation
• Protein Biological Process 3: Blood coagulation
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TF elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for TF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain TF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 296010912
• NCBI GeneID: 2152
• NCBI Accession #: NP_001171567.1
• NCBI GenBank Nucleotide #: NM_001178096.1
• UniProt Accession #: P13726; Q6FHG2; Q86WH4; D3DT47
• Molecular Weight: 33,068 Da
• NCBI Official Full Name: tissue factor isoform 2
• NCBI Official Synonym Full Names: coagulation factor III (thromboplastin, tissue factor)
• NCBI Official Symbol: F3
• NCBI Official Synonym Symbols: TF; TFA; CD142
• NCBI Protein Information: tissue factor
• UniProt Protein Name: Tissue factor
• UniProt Gene Name: F3
• UniProt Synonym Gene Names: TF
• UniProt Entry Name: TF_HUMAN

NCBI Description

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

Function: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.1 PublicationManual assertion based on experiment in:Ref.6