Western Blot (WB)
(The Recombinant immunogen was migrated by 12% SDS-PAGE and transferred to a 0.2 um PVDF membrane.After blocking the membrane in blocking buffer (5% milk powder in 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 0.1% (v/v) Tween 20, the membrane was incubated with biotinylated primary antibody (0.1ug/ml) at 4°C overnight.Enhanced chemiluminescence (ECL) reagents were used to visualize.Recombinant immunogen ()
Thrombopoietin Antigen | TPO antigen
Human Thrombopoietin Recombinant
Purity
>98%, as determined by SDS-PAGE and HPLC
Synonyms
Thrombopoietin; Human Thrombopoietin Recombinant; TPO antigen
Host
Chinese Hamster Ovary Cells
Purity/Purification
>98%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant Thrombopoietinwas lyophilized from a.2 um filtered solution in PBS, pH.
Sequence
MELTELLLVV MLLLTARLTL SSPAPPACDL RVLSKLLRDS HVLHSRLSQC PEVHPLPTPV LLPAVDFSLG EWKTQMEETK AQDILGAVTL LLEGVMAARG QLGPTCLSSL LGQLSGQVRL LLGALQSLLG TQLPPQGRTT AHKDPNAIFL SFQHLLRGKV RFLMLVGGST LCVRRAPPTT AVPSRTSLVL TLNELPNRTS GLLETNFTAS ARTTGSGLLK WQQGFRAKIP GLLNQTSRSL DQIPGYLNRI HELLNGTRGL FPGPSRRTLG APDISSGTSD TGSLPPNLQP GYSPSPTHPP TGQYTLFPLP PTLPTPVVQL HPLLPDPSAP TPTPTSPLLN TSYTHSQNLS QEG
Source Note
Optimized DNA sequence encoding HumanTPO erythropoeitin domain was expressed in Chinese Hamster Ovary cells.
Molecular Weight
Native Human TPO, generated by the proteolytic removal of the signal peptide and propeptide,and has a calculated molecular mass of approximately35 kDa. RecombinantTPO is monomer protein consisting ofamino acid residue subunits, and migrates due to glycosylation as an approximately80 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Biological Activity
The ED(50) was determined by the dose-dependent proliferation of Mo7ecells, was found to be in the range of .0-2.0 ng/ml.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than .1 mg/mL. This solution can then be diluted into other buffers
Preparation and Storage
The lyophilized protein is stable for at least years from date of receipt at -20 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(The Recombinant immunogen was migrated by 12% SDS-PAGE and transferred to a 0.2 um PVDF membrane.After blocking the membrane in blocking buffer (5% milk powder in 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 0.1% (v/v) Tween 20, the membrane was incubated with biotinylated primary antibody (0.1ug/ml) at 4°C overnight.Enhanced chemiluminescence (ECL) reagents were used to visualize.Recombinant immunogen ()
Western Blot (WB)
(The Recombinant immunogen was migrated by 12% SDS-PAGE and transferred to a 0.2 um PVDF membrane.After blocking the membrane in blocking buffer (5% milk powder in 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 0.1% (v/v) Tween 20, the membrane was incubated with biotinylated primary antibody (0.1ug/ml) at 4°C overnight.Enhanced chemiluminescence (ECL) reagents were used to visualize.Recombinant immunogen ()
Product Categories/Family for TPO antigen
NCBI and Uniprot Product Information
Similar Products
Product Notes
The TPO (Catalog #AAA553717) is an Antigen produced from Chinese Hamster Ovary Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MELTELLLVV MLLLTARLTL SSPAPPACDL RVLSKLLRDS HVLHSRLSQC PEVHPLPTPV LLPAVDFSLG EWKTQMEETK AQDILGAVTL LLEGVMAARG QLGPTCLSSL LGQLSGQVRL LLGALQSLLG TQLPPQGRTT AHKDPNAIFL SFQHLLRGKV RFLMLVGGST LCVRRAPPTT AVPSRTSLVL TLNELPNRTS GLLETNFTAS ARTTGSGLLK WQQGFRAKIP GLLNQTSRSL DQIPGYLNRI HELLNGTRGL FPGPSRRTLG APDISSGTSD TGSLPPNLQP GYSPSPTHPP TGQYTLFPLP PTLPTPVVQL HPLLPDPSAP TPTPTSPLLN TSYTHSQNLS QEG. It is sometimes possible for the material contained within the vial of " Thrombopoietin, Antigen" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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