Immunohistochemistry (IHC)
(Figure 2. IHC analysis of Integrin beta 4/ITGB4 using anti-Integrin beta 4/ITGB4 antibody (MBS1753390).Integrin beta 4/ITGB4 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Integrin beta 4/ITGB4 Antibody (MBS1753390) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Rabbit Integrin beta 4/ITGB4 Polyclonal Antibody | anti-ITGB4 antibody
Anti-Integrin beta 4/ITGB4 Antibody
IHC-P: 0.5-1ug/ml|Human|
IF: 4ug/ml|Human|
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of Integrin beta 4/ITGB4 using anti-Integrin beta 4/ITGB4 antibody (MBS1753390).Integrin beta 4/ITGB4 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Integrin beta 4/ITGB4 Antibody (MBS1753390) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of Integrin beta 4/ITGB4 using anti-Integrin beta 4/ITGB4 antibody (MBS1753390).Integrin beta 4/ITGB4 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Integrin beta 4/ITGB4 Antibody (MBS1753390) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 3. IHC analysis of Integrin beta 4/ITGB4 using anti-Integrin beta 4/ITGB4 antibody (MBS1753390).Integrin beta 4/ITGB4 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Integrin beta 4/ITGB4 Antibody (MBS1753390) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 3. IHC analysis of Integrin beta 4/ITGB4 using anti-Integrin beta 4/ITGB4 antibody (MBS1753390).Integrin beta 4/ITGB4 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Integrin beta 4/ITGB4 Antibody (MBS1753390) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 4. IF analysis of Integrin beta 4/ITGB4 using anti- Integrin beta 4/ITGB4 antibody (MBS1753390).Integrin beta 4/ITGB4 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 4μg/mL rabbit anti- Integrin beta 4/ITGB4 Antibody (MBS1753390) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Immunohistochemistry (IHC)
(Figure 4. IF analysis of Integrin beta 4/ITGB4 using anti- Integrin beta 4/ITGB4 antibody (MBS1753390).Integrin beta 4/ITGB4 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 4μg/mL rabbit anti- Integrin beta 4/ITGB4 Antibody (MBS1753390) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Flow Cytometry (FC/FACS)
(Figure 5. Flow Cytometry analysis of Raji cells using anti-Integrin beta 4/ITGB4 antibody (MBS1753390).Overlay histogram showing Raji cells stained with MBS1753390 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin beta 4/ITGB4 Antibody (MBS1753390,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 5. Flow Cytometry analysis of Raji cells using anti-Integrin beta 4/ITGB4 antibody (MBS1753390).Overlay histogram showing Raji cells stained with MBS1753390 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin beta 4/ITGB4 Antibody (MBS1753390,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Western Blot (WB)
(Figure 1. Western blot analysis of Integrin beta 4/ITGB4 using anti-Integrin beta 4/ITGB4 antibody (MBS1753390).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A549 whole cell lysatesLane 2: human PC-3 whole cell lysatesLane 3: human T-47D whole cell lysatesLane 4: human Hela whole cell lysatesLane 5: human SW620 whole cell lysatesLane 6: human CACO-2 whole cell lysatesLane 7: rat ovary tissue lysatesLane 8: mouse ovary tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Integrin beta 4/ITGB4 antigen affinity purified polyclonal antibody (Catalog # MBS1753390) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Integrin beta 4/ITGB4 at approximately 210KD. The expected band size for Integrin beta 4/ITGB4 is at 210KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of Integrin beta 4/ITGB4 using anti-Integrin beta 4/ITGB4 antibody (MBS1753390).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A549 whole cell lysatesLane 2: human PC-3 whole cell lysatesLane 3: human T-47D whole cell lysatesLane 4: human Hela whole cell lysatesLane 5: human SW620 whole cell lysatesLane 6: human CACO-2 whole cell lysatesLane 7: rat ovary tissue lysatesLane 8: mouse ovary tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Integrin beta 4/ITGB4 antigen affinity purified polyclonal antibody (Catalog # MBS1753390) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Integrin beta 4/ITGB4 at approximately 210KD. The expected band size for Integrin beta 4/ITGB4 is at 210KD. )
2. Dajee, M., Lazarov, M., Zhang, J. Y., Cai, T., Green, C. L., Russell, A. J., Marinkovich, M. P., Tao, S., Lin, Q., Kubo, Y., Khavari, P. A. NF-kappa-B blockade and oncogenic Ras trigger invasive human epidermal neoplasia. Nature 421: 639-643, 2003.
3. Geerts, D., Fontao, L., Nievers, M. G., Schaapveld, R. Q. J., Purkis, P. E., Wheeler, G. N., Lane, E. B., Leigh, I. M., Sonnenberg, A. Binding of integrin alpha-6-beta-4 to plectin prevents plectin association with F-actin but does not interfere with intermediate filament binding. J. Cell Biol. 147: 417-434, 1999.
NCBI and Uniprot Product Information
NCBI Description
Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ITGB4: integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Five alternatively spliced isoforms have been described.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion; Receptor, misc.
Chromosomal Location of Human Ortholog: 17q25
Cellular Component: cell surface; hemidesmosome; leading edge; basal plasma membrane; plasma membrane; basement membrane; integrin complex; receptor complex
Molecular Function: protein binding; G-protein-coupled receptor binding; receptor activity
Biological Process: integrin-mediated signaling pathway; skin development; extracellular matrix organization and biogenesis; hemidesmosome assembly; filopodium formation; gut development; cell-matrix adhesion; response to wounding; cell motility involved in cell locomotion; mesodermal cell differentiation; autophagy; cell adhesion
Disease: Epidermolysis Bullosa, Junctional, Non-herlitz Type; Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Junctionalis With Pyloric Atresia
Research Articles on ITGB4
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Product Notes
The ITGB4 itgb4 (Catalog #AAA1753390) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Integrin beta 4/ITGB4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin beta 4/ITGB4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.1-0.25ug/ml|Human, Mouse, Rat| IHC-P: 0.5-1ug/ml|Human| IF: 4ug/ml|Human| FC/FACS/FCM: 1-3ug/1x106 cells|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the ITGB4 itgb4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin beta 4/ITGB4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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