p53 Upregulated Modulator Of Apoptosis Recombinant Protein | PUMA recombinant protein

Recombinant p53 Upregulated Modulator Of Apoptosis (PUMA)

Cat. Num. AAA2033014

• Gene Names: Bbc3; Puma
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: p53 Upregulated Modulator Of Apoptosis; Recombinant p53 Upregulated Modulator Of Apoptosis (PUMA); JFY1; BBC3; BCL2 Binding Component 3; PUMA recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: RWPGG HRSRPRGPRP DGPQPSLSPAQQHLESPVPS APEALAGGPT QAAPGVRVEE EEWAREIGAQ LRRMADDLNAQYERRRQEEQ HRHRPSPWRV
• Sequence Length: 193

• Applicable Applications for PUMA recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 14.5kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Arg76~Val170
• Organism Species: Rat
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 27542559
• NCBI GeneID: 317673
• Molecular Weight: 20,718 Da
• NCBI Official Full Name: PUMA
• NCBI Official Synonym Full Names: Bcl-2 binding component 3
• NCBI Official Symbol: Bbc3
• NCBI Official Synonym Symbols: Puma
• NCBI Protein Information: bcl-2-binding component 3
• UniProt Protein Name: Bcl-2-binding component 3
• UniProt Gene Name: Bbc3
• UniProt Synonym Gene Names: Puma
• UniProt Entry Name: BBC3_RAT

Uniprot Description

PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Cellular Component: endoplasmic reticulum; lysosome; mitochondrion

Molecular Function: ATPase binding; glycoprotein binding; protein binding

Biological Process: apoptosis; brain development; caspase activation; determination of adult life span; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; glial cell differentiation; negative regulation of growth; positive regulation of apoptosis; positive regulation of neuron apoptosis; positive regulation of protein homooligomerization; reduction of endoplasmic reticulum calcium ion concentration; release of cytochrome c from mitochondria; release of sequestered calcium ion into cytosol; response to DNA damage stimulus