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4 results for " Recombinant Human Cytokines Tags" - showing 1-4


Sequence

Endothelial Cell Specific Molecule 1 (ESM1), Active Protein (Cat# AAA2097304)

Full Name
Active Endothelial Cell Specific Molecule 1 (ESM1)
Gene Names
ESM1; endocan
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>92%
Pricing
$225/0.01 mg | $490/0.05 mg | $760/0.1 mg | $940/0.2 mg | $1,830/0.5 mg | $2,710/1 mg | $6,630/5 mg
Activity Data (Lactoferrin (LF), also known as lactotransferrin (LTF), is a multifunctional protein of the transferrin family. Lactoferrin is one of the components of the immune system of the body; it has antimicrobial activity (bacteriocide, fungicide) and is part of the innate defense, mainly at mucoses. In particular, lactoferrin provides antibacterial activity to human infants. Lactoferrin interacts with DNA and RNA, polysaccharides and heparin, and shows some of its biological functions in complexes with these ligands. LTF is known to activate immune cells to produce several cytokines, such as tumor necrosis factor TNF-a, thus, a stimulation assay was conducted to detect the activity of LTF using human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into wells of 24-well plates at a density of 5×106 cells/mL in RPMI-1640 with the addition of various concentrations of recombinant human LTF. After incubation for 20 hours, the concentration of TNF-a in the cell supernatant was detected using an ELISA kit. TNF-a levels in the cell supernatant of spleen cells increased significantly after stimulated with LTF, the data was shown in table 1.)

Lactoferrin (LTF), Active Protein (Cat# AAA2105115)

Full Name
Active Lactoferrin (LTF)
Gene Names
LTF; LF; HLF2; GIG12; HEL110
Applications
WB
Purity
>95%
Pricing
$195/0.01 mg | $390/0.05 mg | $600/0.1 mg | $735/0.2 mg | $1,425/0.5 mg | $2,120/1 mg | $5,215/5 mg
Western Blot (WB) (Figure 1. Western blot analysis of TNFSF18 using anti-TNFSF18 antibody (MBS1750870).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: recombinant human TNFSF18 protein 1ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFSF18 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TNFSF18 at approximately 15KD. The expected band size for TNFSF18 is at 15KD. )

TNFSF18/Gitrl, Polyclonal Antibody (Cat# AAA1750870)

Full Name
Anti-TNFSF18/Gitrl Picoband Antibody
Gene Names
TNFSF18; TL6; AITRL; GITRL; TNLG2A; hGITRL
Reactivity
Human
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Sequence

Interleukin 10, Active Protein (Cat# AAA2122265)

Full Name
Active Interleukin 10 (IL10)
Gene Names
Il10; IL10X
Applications
Cell culture; Activity Assays.
Purity
>95%
Pricing
$250/0.01 mg | $550/0.05 mg | $850/0.1 mg | $1,050/0.2 mg | $2,065/0.5 mg | $3,055/1 mg | $7,480/5 mg
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