Rabbit anti-Human TSC2 Polyclonal Antibody | anti-TSC2 antibody

TSC2, Phosphorylated (Ser1798) (Tuberin, Tuberous Sclerosis 2 Protein, TSC4) (Azide free) (HRP)

Cat. Num. AAA6503944

• Gene Names: TSC2; LAM; TSC4; PPP1R160
• Reactivity: Human
• Applications: ELISA, Dot Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: TSC2; Polyclonal Antibody; Phosphorylated (Ser1798) (Tuberin; Tuberous Sclerosis 2 Protein; TSC4) (Azide free) (HRP); anti-TSC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TSC2 phosphorylated at Ser1798.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TSC2 antibody: ELISA (EIA), Dot Blot (DB)
• Application Notes: ELISA: 1:1,000; Dot Blot: 1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser1798 of human TSC2 (KLH).
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of Phospho-TSC2-S1798 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Dot Blot (DB)

(Dot blot analysis of anti-TSC2-pS1798 Phospho-specific Pab (RB13393) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Product Categories/Family for anti-TSC2 antibody

Antibodies; Tumor Suppressors

References

1. Li, Y., et al., Mol. Cell. Biol. 24(18):7965-7975 (2004). 2. Karbowniczek, M., et al., J. Biol. Chem. 279(29):29930-29937 (2004). 3. Corradetti, M.N., et al., Genes Dev. 18(13):1533-1538 (2004). 4. Birchenall-Roberts, M.C., et al., J. Biol. Chem. 279(24):25605-25613 (2004). 5. Lewis, J.C., et al., J. Med. Genet. 41(3):203-207 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 116256352
• NCBI GeneID: 7249
• NCBI Accession #: NP_000539
• NCBI GenBank Nucleotide #: NM_000548.3
• UniProt Accession #: P49815; O75275; Q4LE71; Q8TAZ1; A7E2E2; B4DIL8; B4DIQ7; B4DRN2; B7Z2B8; C9J378
• Molecular Weight: 25,773 Da
• NCBI Official Full Name: tuberin isoform 1
• NCBI Official Synonym Full Names: tuberous sclerosis 2
• NCBI Official Symbol: TSC2
• NCBI Official Synonym Symbols: LAM; TSC4; PPP1R160
• NCBI Protein Information: tuberin; tuberous sclerosis 2 protein; protein phosphatase 1, regulatory subunit 160
• UniProt Protein Name: Tuberin
• Protein Family: Tuberin
• UniProt Gene Name: TSC2
• UniProt Synonym Gene Names: TSC4
• UniProt Entry Name: TSC2_HUMAN

NCBI Description

Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

TSC2: a product of the tumor suppressor gene TSC2. Tuberin and Hamartin (TSC1) form a tumor suppressor heterodimer that inhibits the mTOR nutrient signaling input. TSC1/TSC2 targets the small G protein Rheb, a novel mediator of the nutrient signaling input to mTOR. Functions as a Rheb GTPase activating protein (GAP). Four alternatively spliced isoforms have been described.

Protein type: Tumor suppressor; GAPs, misc.; Cell cycle regulation; Nuclear receptor co-regulator; GAPs

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: TSC1-TSC2 complex; Golgi apparatus; cytoskeleton; growth cone; membrane; cell soma; perinuclear region of cytoplasm; cytoplasm; dendrite; caveola; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; GTPase activator activity; phosphatase binding

Biological Process: negative regulation of MAP kinase activity; phosphoinositide 3-kinase cascade; regulation of insulin receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; nerve growth factor receptor signaling pathway; protein heterooligomerization; regulation of cell cycle; heart development; negative regulation of insulin receptor signaling pathway; negative regulation of cell size; negative regulation of cell proliferation; protein localization; positive chemotaxis; cell projection organization and biogenesis; protein import into nucleus; regulation of endocytosis; acute-phase response; protein kinase B signaling cascade; cell cycle arrest; protein homooligomerization; negative regulation of epithelial cell proliferation; epidermal growth factor receptor signaling pathway; negative regulation of TOR signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; negative regulation of protein kinase B signaling cascade; endocytosis; vesicle-mediated transport; insulin-like growth factor receptor signaling pathway; glucose import; neural tube closure; insulin receptor signaling pathway; response to hypoxia; innate immune response; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of phosphoinositide 3-kinase cascade

Disease: Lymphangioleiomyomatosis; Tuberous Sclerosis 2