Human ADAM metallopeptidase with thrombospondin type 1 motif, 9 ELISA Kit | ADAMTS9 elisa kit

Human A disintegrin and metalloproteinase with thrombospondin motifs 9, ADAMTS9 ELISA Kit

Cat. Num. AAA937260

• Reactivity: Human
• Synonyms: ADAM metallopeptidase with thrombospondin type 1 motif; 9; Human A disintegrin and metalloproteinase with thrombospondin motifs 9; ADAMTS9 ELISA Kit; Human A disintegrin and metalloproteinase with thrombospondin motifs 9 (ADAMTS9) ELISA kit; FLJ42955; KIAA1312; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; ADAMTS9 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human ADAMTS9. No significant cross-reactivity or interference between human ADAMTS9 and analogues was observed.
• Sequence Length: 1935

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.78 ng/ml - 50 ng/ml.
• Sensitivity: The minimum detectable dose of human ADAMTS9 is typically less than 0.195 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAMTS9 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAMTS9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMTS9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 33624896
• NCBI GeneID: 56999
• NCBI Accession #: NP_891550.1
• NCBI GenBank Nucleotide #: NM_182920.1
• UniProt Accession #: Q9P2N4; Q9NR29; A1L4L0; B7ZVX9; B9ZVN0
• Molecular Weight: 216,491 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 9 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif, 9
• NCBI Official Symbol: ADAMTS9
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 9; ADAM-TS9; ADAMTS-9; ADAM-TS 9; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 9
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 9
• Protein Family: A disintegrin and metalloproteinase with thrombospondin motifs
• UniProt Gene Name: ADAMTS9
• UniProt Synonym Gene Names: KIAA1312; ADAM-TS 9; ADAM-TS9; ADAMTS-9
• UniProt Entry Name: ATS9_HUMAN

NCBI Description

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. Members of the ADAMTS family have been implicated in the cleavage of proteoglycans, the control of organ shape during development, and the inhibition of angiogenesis. This gene is localized to chromosome 3p14.3-p14.2, an area known to be lost in hereditary renal tumors. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAMTS9: Cleaves the large aggregating proteoglycans, aggrecan and versican. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.-; Protease; Motility/polarity/chemotaxis; Extracellular matrix; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p14.1

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; endoplasmic reticulum

Molecular Function: metallopeptidase activity; zinc ion binding; metalloendopeptidase activity

Biological Process: vesicle-mediated transport; protein transport; positive regulation of melanocyte differentiation; multicellular organismal development; glycoprotein catabolic process; proteolysis