Mouse anti-Human Carboxypeptidase A2 Monoclonal Antibody | anti-CPA2 antibody

Anti-Carboxypeptidase A2 Antibody, Mouse Monoclonal

Cat. Num. AAA8103000

• Gene Names: CPA1; CPA
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Protein A
• Synonyms: Carboxypeptidase A2; Monoclonal Antibody; Anti-Carboxypeptidase A2 Antibody; Mouse Monoclonal; Carboxypeptidase A2/CPA2 Antibody; Mouse MAb; carboxypeptidase A2; Anti-CPA2 Antibody; anti-CPA2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone Number: 02
• Specificity: Human Carboxypeptidase A2/CPA2.; No cross-reactivity in ELISA with Human CPE, Human CPB1
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CPA2 antibody: ELISA (EIA) (Cap), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:50-1:200; ELISA (Cap): 1:250-1:2000; ELISA (Cap): This antibody will detect Human Carboxypeptidase A2/CPA2 in ELISA pair set.; In a sandwich ELISA, it can be used as capture antibody when paired with.
• Immunogen: Recombinant Human Carboxypeptidase A2/CPA2 protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Carboxypeptidase A2/CPA2 (rh Carboxypeptidase A2/CPA2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human CPA2 in human pancreas (from 2 donors) with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). Positive staining was localized to pancreatic acinus.)

Related Product Information for anti-CPA2 antibody

Carboxypeptidase A2 (CPA2) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.

References

Catasus, L. et al., 1995. J. Biol. Chem. 270: 6651-6657. Aloy, P. et al., 1998, Biol. Chem. 379: 149-155. Laethem, RM. et al., 1996, Arch. Biochem. Biophys.332: 8-18.

NCBI and Uniprot Product Information

• NCBI GI #: 4502997
• NCBI GeneID: 1357
• NCBI Accession #: NP_001859.1
• NCBI GenBank Nucleotide #: NM_001868.2
• UniProt Accession #: P15085; Q53XU0; Q9BS67; Q9UCF2; A4D1M1
• Molecular Weight: 47,140 Da
• NCBI Official Full Name: carboxypeptidase A1
• NCBI Official Synonym Full Names: carboxypeptidase A1 (pancreatic)
• NCBI Official Symbol: CPA1
• NCBI Official Synonym Symbols: CPA
• NCBI Protein Information: carboxypeptidase A1; pancreatic carboxypeptidase A
• UniProt Protein Name: Carboxypeptidase A1
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPA1
• UniProt Synonym Gene Names: CPA
• UniProt Entry Name: CBPA1_HUMAN

NCBI Description

Three different forms of human pancreatic procarboxypeptidase A have been isolated. This gene encodes a monomeric pancreatic exopeptidase involved in zymogen inhibition. [provided by RefSeq, Jan 2009]

Uniprot Description

CPA1: Carboxypeptidase that catalyzes the release of a C- terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro. Belongs to the peptidase M14 family.

Protein type: Secreted; Secreted, signal peptide; Protease; EC 3.4.17.1

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: extracellular space

Molecular Function: zinc ion binding; metallocarboxypeptidase activity

Biological Process: proteolysis