Chicken HO2 ELISA Kit | HO2 elisa kit

Chicken HO2 (Heme Oxygenase 2) ELISA Kit

Cat. Num. AAA2511566

• Gene Names: HO2; heme oxygenase 2; HO2; T9J22.22; T9J22_22
• Reactivity: Chicken
• Synonyms: HO2; Chicken HO2 (Heme Oxygenase 2) ELISA Kit; HO2 elisa kit

• Reactivity: Chicken
• Specificity: This kit recognizes natural and recombinant Chicken HO2. No significant cross-reactivity or interference between Chicken HO2 and analogues was observed.
• Sequence Length: 314

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HO2 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Chicken HO2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HO2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HO2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HO2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HO2. You can calculate the concentration of HO2 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 334184489
• NCBI GeneID: 817196
• NCBI Accession #: NP_001189611.1
• NCBI GenBank Nucleotide #: NM_001202682.1
• UniProt Accession #: O48722
• Molecular Weight: 34,902 Da
• NCBI Official Full Name: heme oxygenase 2
• NCBI Official Symbol: HO2
• NCBI Official Synonym Symbols: heme oxygenase 2; HO2; T9J22.22; T9J22_22
• NCBI Protein Information: heme oxygenase 2
• UniProt Protein Name: Probable inactive heme oxygenase 2, chloroplastic
• Protein Family: Probable inactive heme oxygenase
• UniProt Gene Name: HO2
• UniProt Synonym Gene Names: AtHO2
• UniProt Entry Name: HMOX2_ARATH

NCBI Description

Encodes a heme oxygenase-like protein lacking the conserved histidine residue at the active site that is usually involved in heme-iron coordination. It is unable to bind and degrade heme. Mutant analyses suggest a role in photomorphogenesis. The protein can bind the heme precursor, proto IX, which could be biologically significant and point to a role in the regulation of the two tetrapyrrole biosynthetic pathways.

Uniprot Description

Probable inactive heme oxygenase. Binds protoporphyrin IX, a precursor for both heme and chlorophyll biosynthesis. Plays a minor role in phytochrome assembly and photomorphogenesis.