Canine Heme Oxygenase 2, Decycling ELISA Kit | HO2 elisa kit

Canine Heme Oxygenase 2, Decycling ELISA Kit

Cat. Num. AAA737104

• Gene Names: HO2; heme oxygenase 2; HO2; T9J22.22; T9J22_22
• Reactivity: Canine
• Synonyms: Heme Oxygenase 2; Decycling; Canine Heme Oxygenase 2; Decycling ELISA Kit; HO2 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HO2 elisa kit

Principle of the Assay: HO2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HO2 antibody and an HO2-HRP conjugate. The assay sample and buffer are incubated together with HO2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HO2 concentration since HO2 from samples and HO2-HRP conjugate compete for the anti-HO2 antibody binding site. Since the number of sites is limited, as more sites are occupied by HO2 from the sample, fewer sites are left to bind HO2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HO2 concentration in each sample is interpolated from this standard curve.

Intended Uses: This HO2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine HO2. This ELISA kit for research use only!

Product Categories/Family for HO2 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 334184489
• NCBI GeneID: 817196
• NCBI Accession #: NP_001189611.1
• NCBI GenBank Nucleotide #: NM_001202682.1
• Molecular Weight: 34,902 Da
• NCBI Official Full Name: heme oxygenase 2
• NCBI Official Symbol: HO2
• NCBI Official Synonym Symbols: heme oxygenase 2; HO2; T9J22.22; T9J22_22
• NCBI Protein Information: heme oxygenase 2
• UniProt Protein Name: Probable inactive heme oxygenase 2, chloroplastic
• Protein Family: Probable inactive heme oxygenase
• UniProt Gene Name: HO2
• UniProt Synonym Gene Names: At2g26550; T9J22.22; AtHO2
• UniProt Entry Name: HMOX2_ARATH

NCBI Description

Encodes a heme oxygenase-like protein lacking the conserved histidine residue at the active site that is usually involved in heme-iron coordination. It is unable to bind and degrade heme. Mutant analyses suggest a role in photomorphogenesis. The protein can bind the heme precursor, proto IX, which could be biologically significant and point to a role in the regulation of the two tetrapyrrole biosynthetic pathways.

Uniprot Description

Probable inactive heme oxygenase. Binds protoporphyrin IX, a precursor for both heme and chlorophyll biosynthesis. Plays a minor role in phytochrome assembly and photomorphogenesis.