Mouse anti-Human TLR6 Monoclonal Antibody | anti-TLR6 antibody

NALE Monoclonal Antibody to TLR6 (Clone: ABM1B50) (No Azide Low Endotoxin)

Cat. Num. AAA668948

• Gene Names: TLR6; CD286
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Azide Free, Purified; Protein G Chromatography
• Synonyms: TLR6; Monoclonal Antibody; NALE Monoclonal Antibody to TLR6 (Clone: ABM1B50) (No Azide Low Endotoxin); anti-TLR6 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone Number: ABM1B50
• Purity/Purification: Azide Free, Purified; Protein G Chromatography
• Form/Format: 100 ug in 200 ul PBS containing 0.05% BSA.
• Sequence Length: 796

• Applicable Applications for anti-TLR6 antibody: Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: WB analysis: 2-4 ug/ml; FC/FACS analysis: 0.5-1 ug/10^6 cells
• Antigen: TLR6
• Immunogen Information: A partial length recombinant TLR6 protein (amino acids 280-512) was used as the immunogen for this antibody.
• Conjugation: Unconjugated
• Preparation and Storage: Store the antibody at 4 degree C; stable for 6 months.; For long-term storage; store at -20 degree C.; Avoid repeated freeze and thaw cycles.

Western Blot (WB)

(Fig-1: Western blot analysis of TLR6. Anti- TLR6 antibody (Clone: ABM1B50) was used at 2 ug/ml on Jurkat lysate.)

Flow Cytometry (FC/FACS)

(Fig-2: Intracellular flow analysis of TLR6 in THP-1 cells using 0.5 ug/10^6 cells of TLR6 antibody (Clone: ABM1B50). Green represents isotype control; red represents anti-TLR6 antibody. Goat anti-Mouse PE conjugate was used as secondary antibody.)

Related Product Information for anti-TLR6 antibody

TLR6 (Toll-Like Receptors 6) is a member of the TLR (Toll-like receptor) family that plays a fundamental role in pathogen recognition and activation of innate immunity. TLR6 forms heterodimers with TLR1 and TLR2, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens and protection from immune sequelae related to infection with these pathogens. TLR2 in co-operation with TLR6 trigger inflammatory responses via activation of NF-kappaB, which regulates the transcription of inflammatory molecules. TLR6 has emerged as the most potent activator for ZNPs (Zinc Oxide Nanoparticles)-induced inflammatory responses. Upon silencing, TLR6 significantly inhibited the pro-inflammatory cytokine levels, reactive nitrogen species generation and inducible nitric oxide synthase expression.

Product Categories/Family for anti-TLR6 antibody

TLR and Innate Immunity

NCBI and Uniprot Product Information

• NCBI GI #: 20143971
• NCBI GeneID: 10333
• NCBI Accession #: NP_006059.2
• NCBI GenBank Nucleotide #: NM_006068.4
• UniProt Accession #: Q9Y2C9; Q2NKL3; B3Y640; B6CH35; B6RFS4; B6RFS5
• Molecular Weight: 55,828 Da
• NCBI Official Full Name: toll-like receptor 6
• NCBI Official Synonym Full Names: toll like receptor 6
• NCBI Official Symbol: TLR6
• NCBI Official Synonym Symbols: CD286
• NCBI Protein Information: toll-like receptor 6
• UniProt Protein Name: Toll-like receptor 6
• UniProt Gene Name: TLR6

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor functionally interacts with toll-like receptor 2 to mediate cellular response to bacterial lipoproteins. A Ser249Pro polymorphism in the extracellular domain of the encoded protein may be associated with an increased of asthma is some populations.[provided by RefSeq, Jan 2011]

Uniprot Description

Participates in the innate immune response to Gram-positive bacteria and fungi. Specifically recognizes diacylated and, to a lesser extent, triacylated lipopeptides (PubMed:20037584). In response to diacylated lipopeptides, forms the activation cluster TLR2:TLR6:CD14:CD36, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2 (PubMed:11441107). In complex with TLR4, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion (PubMed:11441107, PubMed:20037584).