ACE2/HEK293 Cell Line

ACE2/HEK293 Stable Cell Line

Cat. Num. AAA669482

• Applications: Functional Assay
• Synonyms: ACE2/HEK293; ACE2/HEK293 Stable Cell Line; ACE2/HEK293 cell line

• Form/Format: Each vial contains 2~3 x 10^6 cells in 1ml of 90% FBS + 10% DMSO
• Sequence: MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF

• Applicable Applications for ACE2/HEK293 cell line: Functional Assay (FA)
• Application Notes: Screen for antibodies of human ACE2 through Flow Cytometry. Protein binding study.
• Culture Conditions: Cells should be grown at 3 7 degree C with 5% CO2 using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supplemented with 10% heat-inactivated FBS supplemented with 10% FBS and 1% Pen/Strep, plus 250ug/ml of Hygromycin.; It is recommended to quickly thaw the frozen cells upon receipt or from liquid nitrogen in a 37 degree C water-bath, transfer to a tube containing 10 ml of growth medium without Hygromycin, spin down cells, resuspend cells in pre-warmed growth medium without Hygromycin, transfer resuspended cells to T25 flask and culture in 3 7 degree C-CO2 incubator.; Leave the T25 flask in the incubator for 1~2 days without disturbing or changing the medium until cells completely recover viability and become adherent. Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation. At first passage, switch to growth medium containing Hygromycin. Cells should be split before they reach complete confluence.; To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly. To achieve satisfactory results, cells should not be passaged over 16 times.
• Notes: The ACE2 Stable cell line is available in three cell line formats: ACE2/CHO-K1 (MBS669481), ACE2/HEK293 (MBS669482), ACE2/VERO (MBS669483)
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Immediately upon receipt, store in liquid nitrogen.

Testing Data

(Fig-1: Binding of biotinylated SARS-Cov-2 Spike RBD protein to human ACE2 in the ACE2/HEK293 stable cell line. ACE2/HEK293 cells were probed with different amounts of biotinylated SARS-Cov-2 Spike RBD protein and analyzed through In-Cell ELISA using HRP-Streptavidin for detection.)

Testing Data

(Fig-2: Detection of ACE2 expression in ACE2/HEK293 cells by flow cytometry. ACE2/HEK293 cells were probed with biotinylated SARS-Cov-2 Spike RBD protein at 1 x 10^5 cells per 1 ug protein on ice for 2 hours. Cells were washed with flow buffer and then incubated with PE-labeled Streptavidin on ice for 20 min. Cell were washed and then analyzed by flow cytometry.)

Testing Data

(Fig-3: Binding of biotinylated SARS-Cov-2 Spike RBD protein to human ACE2 in the ACE2/HEK293 stable cell line. ACE2/HEK293 cells were probed with different amounts of biotinylated SARS-Cov-2 Spike RBD protein and analyzed by flow cytometry through fluorescent-labeled Streptavidin detection.)

Testing Data

(Fig-4: Genomic PCR analysis. Genomic DNAs were purified from ACE2/HEK293 stable cells and parental HEK293 cells, and used as the PCR templates. PCR was performed using the target gene construct-specific primer sets, and the plasmid DNA of target gene construct was used as positive control.)

Testing Data

(Fig-5: Cell Surface staining of human ACE2 in the ACE2/HEK293 stable cell line. ATTO 488-conjugated anti-hACE2 antibody (clone AC18F) was used at 1ug/ 1x10^6 cells (Red). ATTO 488-conjugated mouse IgG1 was used as isotype control at 1ug/ 1x10^6 cells (Green).)

Related Product Information for ACE2/HEK293 cell line

ACE2/HEK293 Stable Cell Line is a stably transfected HEK293 cell line which expresses human angiotensin-converting enzyme 2 (ACE2). ACE2 is a type I transmembrane metalloenzyme located on the outer surface of endothelial cells in the lung, arteries, heart, kidney and intestines. ACE2 cleaves the carboxyl-terminal amino acid phenylalanine from angiotensin II and hydrolyses it into the vasodilator angiotensin. ACE2 also serves as an entry receptor for some coronaviruses including HCoV-NL63, SARS-CoV and SARS-CoV-2 as the virus that causes COVID-19.

Product Categories/Family for ACE2/HEK293 cell line

Cell Lines; Stable Cell Lines

NCBI and Uniprot Product Information