CDO Recombinant Protein | CDON recombinant protein

CDO Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121030

• Purity: >65% as determined by SDS-PAGE
• Synonyms: CDO; CDO Protein; Human; Recombinant (His Tag); Human CDON/CDO Protein (His Tag); CDON1 Protein; HPE11 Protein; ORCAM Protein; cell adhesion associated; oncogene regulated; CDON recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >65% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp963

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asp 26
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CDON (AAI14436.1) extracellular domain (Met 1-Asp 963) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CDON recombinant protein

Background: Cell adhesion molecule-related, down-regulated by oncogenes (CDON), also known as CDO, is an Ig superfamily member, is a component of a cell surface receptor that positively regulates skeletal myogenesis. Brother of CDO (BOC) is a cell surface receptor that derives its name from the structurally related protein, CDON. They are components of a cell surface receptor that positively regulates myogenesis in vitro. Expression of Cdo and Boc in myoblast cell lines is downregulated by the ras oncogene, and forced re-expression of either Cdo or Boc can override ras-induced inhibition of myogenic differentiation. CDO and BOC play a role in the inverse relationship between differentiation and transformation of cells in the skeletal muscle lineage. CDON binds to Bnip-2 and JLP, scaffold proteins for Cdc42 and p38alpha/beta MAPK, respectively. The Bnip-2/Cdc42 and JLP/p38alpha/beta complexes associate in a CDON-dependent manner, resulting in Bnip-2/Cdc42-dependent p38alpha/beta activation and stimulation of cell differentiation. It is proposed that CDO mediates, at least in part, the effects of cell-cell interactions between muscle precursors that are critical in myogenesis.

References

Kang JS, et al. (1998) CDO, a robo-related cell surface protein that mediates myogenic differentiation. J Cell Biol. 143(2): 403-13.Wegorzewska M, et al. (2003) Overexpression of the immunoglobulin superfamily members CDO and BOC enhances differentiation of the human rhabdomyosarcoma cell line RD. Mol Carcinog. 37(1): 1-4.Cole F, et al. (2003) Microform holoprosencephaly in mice that lack the Ig superfamily member Cdon. Curr Biol. 13(5): 411-5.Jehee FS, et al. (2006) Mutational screening of FGFR1, CER1, and CDON in a large cohort of trigonocephalic patients. Cleft Palate Craniofac J. 43(2): 148-51.Kavran JM, et al. (2010) All mammalian Hedgehog proteins interact with cell adhesion molecule, down-regulated by oncogenes (CDO) and brother of CDO (BOC) in a conserved manner. J Biol Chem. 285(32): 24584-90.Lu M, et al. (2010) N-cadherin ligation, but not Sonic hedgehog binding, initiates Cdo-dependent p38alpha/beta MAPK signaling in skeletal myoblasts. Proc Natl Acad Sci U S A. 107(9): 4212-7.

NCBI and Uniprot Product Information

• Molecular Weight: The recombinant human CDON consists of 949 amino acids and has a calculated molecular mass of 103kDa. It migrates as an approximately 125kDa band in SDS-PAGE under reducing conditions.
• Protein Family: Cell adhesion molecule-related/down-regulated by oncogenes