Western Blot (WB)
(Western blot analysis of eNOS expression in rat heart (A) whole cell lysates.)
Mouse eNOS Monoclonal Antibody | anti-NOS3 antibody
Anti-eNOS Antibody
Immunohistochemistry: (1/200 - 1/500)
Western Blot (WB)
(Western blot analysis of eNOS expression in rat heart (A) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of eNOS expression in rat heart (A) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of eNOS staining in mouse colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of eNOS staining in mouse colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
Uniprot Description
eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.
Protein type: Amino Acid Metabolism - arginine and proline; Oxidoreductase; EC 1.14.13.39
Chromosomal Location of Human Ortholog: 7q36
Cellular Component: caveola; cytoplasm; cytoskeleton; cytosol; Golgi membrane; nucleus; plasma membrane
Molecular Function: actin monomer binding; cadmium ion binding; calmodulin binding; FAD binding; FMN binding; heme binding; iron ion binding; NADP binding; nitric-oxide synthase activity; protein binding
Biological Process: angiogenesis; arginine catabolic process; blood coagulation; blood vessel remodeling; cell redox homeostasis; endothelial cell migration; in utero embryonic development; lipopolysaccharide-mediated signaling pathway; lung development; mitochondrion organization and biogenesis; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of cell proliferation; negative regulation of hydrolase activity; negative regulation of muscle hyperplasia; negative regulation of potassium ion transport; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; nitric oxide metabolic process; ovulation from ovarian follicle; positive regulation of angiogenesis; positive regulation of guanylate cyclase activity; positive regulation of vasodilation; regulation of blood pressure; regulation of blood vessel size; regulation of nitric-oxide synthase activity; regulation of sodium ion transport; regulation of systemic arterial blood pressure by endothelin; regulation of the force of heart contraction by chemical signal; removal of superoxide radicals; response to heat; smooth muscle hyperplasia; vascular endothelial growth factor receptor signaling pathway
Disease: Alzheimer Disease; Hypertension, Essential; Preeclampsia/eclampsia 1; Stroke, Ischemic
Research Articles on NOS3
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Product Notes
The NOS3 nos3 (Catalog #AAA8241498) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-eNOS Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's eNOS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/2000)Immunohistochemistry: (1/200 - 1/500). Researchers should empirically determine the suitability of the NOS3 nos3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eNOS, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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