CDK9/CyclinK Recombinant Protein | CDK9 recombinant protein

CDK9/CyclinK, Active

Cat. Num. AAA516727

• Gene Names: CCNK; CPR4
• Applications: Western Blot
• Synonyms: CDK9/CyclinK; Active; CDC2L4; C-2k; TAK; PITALRE; CCNK; CPR4; MGC9113; CDK9 recombinant protein

• Host: Sf9 insect cells
• Form/Format: Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

• Applicable Applications for CDK9 recombinant protein: Kinase Assay, Western Blot (WB)
• Tag: GST
• Species: Human
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Activity

(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)

SDS-Page

(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)

Related Product Information for CDK9 recombinant protein

Recombinant full-length human CDK9 and CyclinK proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession numbers for CDK9 and CyclinK are NM_001261 and, NM_003858, respectively.
CDK9/CyclinK is a member of the cyclin-dependent protein kinase (CDK) family. CDK9 is closely related to cdc28 and cdc2 and is an important regulator of the cell cycle (1). CDK9 is a component of the multiprotein complex TAK/P-TEFbeta. CDK9 can modulate RNA polymerase II-directed transcription by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. CDK9 forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. CDK9 also interacts with the HIV-1 Tat protein which suggested a possible involvement of this protein in AIDS (2).

Product Categories/Family for CDK9 recombinant protein

Protein Kinases; Cancer; Cell Cycle; Ser/Thr Kinases

References

1. Yang, Z. et al: The 7SK small nuclear RNA inhibits the CDK9/cyclin T1 kinase to control transcription. Nature, 2001; 414: 317-322.
2. Bullrich, F. et al: Chromosomal mapping of members of the cdc2 family of protein kinases, cdk3, cdk6, PISSLRE, and PITALRE, and a cdk inhibitor, p27-Kip1, to regions involved in human cancer. Cancer Res. 1995; 55: 1199-1205.

NCBI and Uniprot Product Information

• NCBI GI #: 16198507
• NCBI GeneID: 8812
• NCBI Accession #: BC015935
• NCBI GenBank Nucleotide #: NM_001261
• UniProt Accession #: O75909; Q59FT6; Q86U16; Q96B63; Q9NNY9
• Molecular Weight: 67 kDa
• NCBI Official Full Name: Homo sapiens cyclin K, mRNA
• NCBI Official Synonym Full Names: cyclin K
• NCBI Official Symbol: CCNK
• NCBI Official Synonym Symbols: CPR4
• NCBI Protein Information: cyclin-K
• UniProt Protein Name: Cyclin-K
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CCNK
• UniProt Synonym Gene Names: CPR4
• UniProt Entry Name: CCNK_HUMAN

NCBI Description

The protein encoded by this gene is a member of the transcription cyclin family. These cyclins may regulate transcription through their association with and activation of cyclin-dependent kinases (CDK) that phosphorylate the C-terminal domain (CTD) of the large subunit of RNA polymerase II. This gene product may play a dual role in regulating CDK and RNA polymerase II activities. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: May play a role in transcriptional regulation. In vitro, is associated with a kinase activity toward both RNA polymerase II C-terminal domain and CDK2 (CAK). Ref.9

Subunit structure: Part of a cyclin-kinase pair in the RNA polymerase II holoenzyme. Binds to CDK9.

Tissue specificity: Ubiquitously expressed. Highest levels in testis.

Sequence similarities: Belongs to the cyclin family. Cyclin C subfamily.