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CDK10/CyclinM Recombinant Protein | CDK10 recombinant protein
CDK10/CyclinM Protein
Synonyms
CDK10/CyclinM; CDK10/CyclinM Protein; CDK10: ALSAS; PISSLRE CyclinM: CCNQ; CycM; FAM58A; CDK10 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glycerol.
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
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(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for CDK10 recombinant protein
Recombinant full-length human CDK10 and CyclinM proteins were co-expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. The gene accession numbers for CDK 10 and CyclinM are NM_052988 and, NM_152274, respectively.
Cyclin-dependent kinase 10 (CDK10) belongs to cdc2 family of kinases and regulates the cell cycle (1). Cyclin M, the only conventional cyclin partner, protects CDK10 from ubiquitin mediated degradation (2). CDK10/cyclinM positively regulates the degradation of ETS2 through phosphorylation of two neighboring serines. EST2 is a transcription factor that plays key roles in cancer and development. CDK10/CyclinM is also known to repress ciliogenesis and to maintain actin cytoskeletan through the phoshorylation of the PKN2 protein kinase and the control of RhoA stability (3).
Cyclin-dependent kinase 10 (CDK10) belongs to cdc2 family of kinases and regulates the cell cycle (1). Cyclin M, the only conventional cyclin partner, protects CDK10 from ubiquitin mediated degradation (2). CDK10/cyclinM positively regulates the degradation of ETS2 through phosphorylation of two neighboring serines. EST2 is a transcription factor that plays key roles in cancer and development. CDK10/CyclinM is also known to repress ciliogenesis and to maintain actin cytoskeletan through the phoshorylation of the PKN2 protein kinase and the control of RhoA stability (3).
Product Categories/Family for CDK10 recombinant protein
References
1. Li, S. et. al: The cdc-2-related kinase, PISSLRE, is essential for cell growth and acts in G2 phase of the cell cycle. Cancer Res. 55:3992-3995, 1995.
2. Khanal, P. et. al: Proyl isomerase Pin1 facilitates ubiquitin-mediated degradation of cyclin-dependent kinase 10 to induce tamoxifen resistance in breast cancer cells. Oncogene. 31:3845-56, 2012.
3. Guen, V. J. et. al: The awakening of the CDK10/Cyclin M protein kinase. Oncotarget. 8(30), 50174-50186, 2017.
2. Khanal, P. et. al: Proyl isomerase Pin1 facilitates ubiquitin-mediated degradation of cyclin-dependent kinase 10 to induce tamoxifen resistance in breast cancer cells. Oncogene. 31:3845-56, 2012.
3. Guen, V. J. et. al: The awakening of the CDK10/Cyclin M protein kinase. Oncotarget. 8(30), 50174-50186, 2017.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67kDa/52kDa
Protein Family
Similar Products
Product Notes
The CDK10 (Catalog #AAA516730) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CDK10/CyclinM, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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