Testing Data
CDK1/CyclinB1 Recombinant Protein | CDK1 recombinant protein
CDK1/CyclinB1, Active
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Scientific Background: CDK1 or Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes (1). Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated (2).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Uniprot Description
CDK1: a protein kinase of the CDK family. Catalytic subunit of the conserved protein complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions. Mitotic cyclins stably associate with this protein and function as regulatory subunits. Its activity is controlled by cyclin availability and phosphorylation through the cell cycle. Activated in many cancers including colon, liver and breast. The T isoform, which lacks a regulatory region, is expressed in breast cancer. Inhibition in cancer cells may drive cells into apoptosis. May also drive cell migration. Inhibitors: BMS-265246, BMS-265246-01. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: CDK family; CDK/CDK1 subfamily; CDK1 subfamily; CMGC group; Cell cycle regulation; EC 2.7.11.22; EC 2.7.11.23; Kinase, protein; Motility/polarity/chemotaxis; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 10q21.2
Cellular Component: centrosome; cyclin-dependent protein kinase holoenzyme complex; cytosol; endoplasmic reticulum membrane; membrane; midbody; mitochondrial matrix; mitochondrion; nuclear chromosome, telomeric region; nucleoplasm; nucleus; spindle microtubule
Molecular Function: cyclin binding; cyclin-dependent protein kinase activity; histone kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity; RNA polymerase subunit kinase activity
Biological Process: activation of MAPK activity; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell migration; centrosome cycle; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA repair; DNA replication; epithelial cell differentiation; G2/M transition of mitotic cell cycle; microtubule cytoskeleton organization and biogenesis; mitotic nuclear envelope disassembly; negative regulation of apoptosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; pronuclear fusion; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; protein deubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of embryonic development; regulation of gene expression; regulation of meiotic cell cycle; regulation of Schwann cell differentiation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle
Research Articles on CDK1
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Product Notes
The CDK1 cdk1 (Catalog #AAA515463) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Full Length. AAA Biotech's CDK1/CyclinB1 can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the CDK1 cdk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDK1/CyclinB1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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