Mouse CD325 ELISA Kit | CDH2 elisa kit

Mouse CD325 ELISA Kit

Cat. Num. AAA8244692

• Gene Names: Cdh2; CDHN; Ncad; N-CAD
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: CD325; Mouse CD325 ELISA Kit; CDHN; NCAD; Cadherin-2; CDw325; Neural cadherin; N-cadherin; CDH2 elisa kit

• Reactivity: Mouse
• Specificity: The Mouse CD325 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CD325 proteins within the range of 780 pg/ml - 50000 pg/ml.
• Sequence Length: 906

• Applicable Applications for CDH2 elisa kit: Sandwich ELISA (EIA)
• Samples: Cell Culture Supernatants, Serum, Plasma.
• Sensitivity: 20 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve

Related Product Information for CDH2 elisa kit

Principle of the Assay||The Mouse CD325 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD325 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CD325 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD325 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD325 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD325 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Cadherin-2 (CDH2), also known as neural cadherin (NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.

NCBI and Uniprot Product Information

• NCBI GI #: 341940300
• NCBI GeneID: 12558
• UniProt Accession #: P15116; Q64260; Q6GU11
• Molecular Weight: 99,796 Da
• NCBI Official Full Name: Cadherin-2
• NCBI Official Synonym Full Names: cadherin 2
• NCBI Official Symbol: Cdh2
• NCBI Official Synonym Symbols: CDHN; Ncad; N-CAD
• NCBI Protein Information: cadherin-2
• UniProt Protein Name: Cadherin-2
• Protein Family: Cadherin
• UniProt Gene Name: Cdh2
• UniProt Synonym Gene Names: N-cadherin
• UniProt Entry Name: CADH2_MOUSE

NCBI Description

This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion. The encoded preproprotein undergoes proteolytic processing to generate a mature protein. Mice lacking the encoded protein exhibit severe developmental defects resulting in embryonic death. [provided by RefSeq, Oct 2015]

Uniprot Description

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence (PubMed:24952463). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.