SDS-Page
CD73/NT5E Recombinant Protein | NT5E recombinant protein
Human CellExp CD73/NT5E, Human recombinant
Purity
>85% as determined by SDS-PAGE.
Synonyms
CD73/NT5E; Human CellExp CD73/NT5E; Human recombinant; CD73; NT5E; 5'-Nucleotidase; 5'-NT; NT5; NTE; NT5E recombinant protein
Host
HEK293 cells
Purity/Purification
>85% as determined by SDS-PAGE.
Form/Format
Lyophilized powder. Lyophilized from 0.22 um filtered solution in 20mM Tris, 120mMNaCl, 4mM CaCl2, pH7.5. Normally trehalose is added as protectant before lyophilization.
Sequence
aaTrp27-Ser549
Gene Source
Human
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Reconstitution Instruction
Reconstitute in sterile deionized water, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. It is recommended to store at store at -20 degree C.
Tag Note
This protein carries a polyhistidine tag at the C-terminus.
Preparation and Storage
Store at -20 degree C
Centrifuge the vial prior to opening.
Shelf life: ~12 months
Centrifuge the vial prior to opening.
Shelf life: ~12 months
SDS-Page
SDS-Page
Related Product Information for NT5E recombinant protein
5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain. Enzyme that convert AMP to adenosine
Product Categories/Family for NT5E recombinant protein
NCBI and Uniprot Product Information
Similar Products
Product Notes
The NT5E (Catalog #AAA8400003) is a Recombinant Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: aaTrp27-Se r549. It is sometimes possible for the material contained within the vial of "CD73/NT5E, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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