CD59a recombinant protein

CD59a Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8122949

• Gene Names: Cd59a; Cd59; AA987121; protectin
• Purity: >92% as determined by SDS-PAGE
• Synonyms: CD59a; CD59a Protein; Mouse; Recombinant (hFc Tag); Mouse CD59a/Protectin/MAC-IP Protein (Fc Tag); AA987121 Protein; Cd59 Protein; protectin Protein; RP24-297H17.1 Protein; CD59a antigen; CD59a recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys95

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 24
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse CD59a (NP_001104530.1)(Met 1-Lys 95),without the pro peptide, was fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD59a recombinant protein

Background: Protectin, a complement regulatory protein, also known as CD59, or MIRL (membrane inhibitor of reactive lysis) is a small protein that inhibits the complement membrane attack complex by binding C5b678 and preventing C9 from binding and polymerizing. The amino-terminal 25 amino acids represented a typical signal peptide sequence and the carboxy-terminal hydrophobic amino acids were characteristic for phosphatidylinositol-anchored proteins. It was found that the CD59/Protectin antigen is a small protein sometimes associated with larger components (45 and 80 kD) in urine. CD59/Protectin antigen was released from the surface of transfected COS cells by phosphatidylinositol-specific phospholipase C, demonstrating that it is attached to the cell membrane by means of a glycolipid anchor; it is therefore likely to be absent from the surface of affected erythrocytes in the disease paroxysmal nocturnal hemoglobinuria.

References

Huang Y, et al. (2006) Defining the CD59-C9 binding interaction. J Biol Chem. 281 (37): 27398-404.Sawada R, et al. (1990) Isolation and expression of the full-length cDNA encoding CD59 antigen of human lymphocytes. DNA Cell Biol. 9(3): 213-20.Philbrick WM, et al. (1990) The CD59 antigen is a structural homologue of murine Ly-6 antigens but lacks interferon inducibility. Eur J Immunol. 20(1): 87-92.

NCBI and Uniprot Product Information

• NCBI GI #: 161484616
• NCBI GeneID: 12509
• NCBI Accession #: NP_001104530.1
• NCBI GenBank Nucleotide #: NM_001111060.2
• UniProt Accession #: O55186; Q542R7
• Molecular Weight: 13,648 Da
• NCBI Official Full Name: CD59A glycoprotein
• NCBI Official Synonym Full Names: CD59a antigen
• NCBI Official Symbol: Cd59a
• NCBI Official Synonym Symbols: Cd59; AA987121; protectin
• NCBI Protein Information: CD59A glycoprotein
• UniProt Protein Name: CD59A glycoprotein
• Protein Family: CD59A glycoprotein
• UniProt Gene Name: Cd59a
• UniProt Synonym Gene Names: Cd59; MAC-IP; MACIF
• UniProt Entry Name: CD59A_MOUSE

Uniprot Description

CD59A: Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore.

Cellular Component: external side of plasma membrane; membrane; plasma membrane

Molecular Function: complement binding

Biological Process: cell surface receptor linked signal transduction; negative regulation of activation of membrane attack complex; negative regulation of angiogenesis; negative regulation of complement activation; negative regulation of vascular endothelial growth factor receptor signaling pathway