Mouse CD59A glycoprotein ELISA Kit | Cd59a elisa kit

Mouse CD59A glycoprotein ELISA Kit

Cat. Num. AAA288233

• Gene Names: Cd59a; Cd59; AA987121; protectin
• Reactivity: Mouse
• Synonyms: CD59A glycoprotein; Mouse CD59A glycoprotein ELISA Kit; Cd59a elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse CD59A glycoprotein. No significant cross-reactivity or interference was observed.
• Sequence Length: 123

• Assay Type: Sandwich
• Detection Range: 78-5000 pg/mL
• Sensitivity: 39 pg/mL
• Intra-Assay CV: <=8.3%
• Inter-Assay CV: <=10.1%
• Recovery: 93%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Cd59a elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD59A glycoprotein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CD59A glycoprotein and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CD59A glycoprotein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CD59A glycoprotein in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 161484616
• NCBI GeneID: 12509
• NCBI Accession #: NP_001104530.1
• NCBI GenBank Nucleotide #: NM_001111060.2
• UniProt Accession #: O55186; Q542R7
• Molecular Weight: 13,648 Da
• NCBI Official Full Name: CD59A glycoprotein
• NCBI Official Synonym Full Names: CD59a antigen
• NCBI Official Symbol: Cd59a
• NCBI Official Synonym Symbols: Cd59; AA987121; protectin
• NCBI Protein Information: CD59A glycoprotein
• UniProt Protein Name: CD59A glycoprotein
• Protein Family: CD59A glycoprotein
• UniProt Gene Name: Cd59a
• UniProt Synonym Gene Names: Cd59; MAC-IP; MACIF
• UniProt Entry Name: CD59A_MOUSE

Uniprot Description

CD59A: Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore.

Cellular Component: external side of plasma membrane; membrane; plasma membrane

Molecular Function: complement binding

Biological Process: cell surface receptor linked signal transduction; negative regulation of activation of membrane attack complex; negative regulation of angiogenesis; negative regulation of complement activation; negative regulation of vascular endothelial growth factor receptor signaling pathway