SDS-Page
CD58 recombinant protein
CD58, 29-215aa, Human, His-tag, Baculovirus
Gene Names
CD58; ag3; LFA3; LFA-3
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
CD58; 29-215aa; Human; His-tag; Baculovirus; Lymphocyte function-associated antigen 3 isoform 1; ag3; LFA-3; LFA3; CD58 recombinant protein
Host
Baculovirus
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
Concentration
1mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence
ADPFSQQIYG VVYGNVTFHV PSNVPLKEVL WKKQKDKVAE LENSEFRAFS SFKNRVYLDT VSGSLTIYNL TSSDEDEYEM ESPNITDTMK FFLYVLESLP SPTLTCALTN GSIEVQCMIP EHYNSHRGLI MYSWDCPMEQ CKRNSTSIYF KMENDLPQKI QCTLSNPLFN TTSSIILTTC IPSSGHSRHR HHHHHH
Sequence Length
250
Applicable Applications for CD58 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1ug of protein (determined by LAL method)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for CD58 recombinant protein
CD58, also known as lymphocyte function-associated antigen 3 isoform 1, is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. It binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. It is an important regulator of immune cell function which occurs as both surface-associated and soluble forms. It is associated with increased risk for multiple sclerosis. Recombinant human CD58, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for CD58 recombinant protein
References
Abdul Razak FR., et al. (2016) Genes Immun. 17:363-366.; Kirby AC., et al. (2000) Cell Adhes Commun. 7:453-464.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22.5kDa (196aa) 28-40kDa (SDS-PAGE under reducing conditions)
NCBI Official Full Name
lymphocyte function-associated antigen 3 isoform 1
NCBI Official Synonym Full Names
CD58 molecule
NCBI Official Symbol
CD58
NCBI Official Synonym Symbols
ag3; LFA3; LFA-3
NCBI Protein Information
lymphocyte function-associated antigen 3
UniProt Protein Name
Lymphocyte function-associated antigen 3
Protein Family
UniProt Gene Name
CD58
UniProt Synonym Gene Names
LFA3; Ag3
NCBI Description
This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]
Uniprot Description
Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.
Research Articles on CD58
Similar Products
Product Notes
The CD58 cd58 (Catalog #AAA206743) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's CD58 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the CD58 cd58 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ADPFSQQIYG VVYGNVTFHV PSNVPLKEVL WKKQKDKVAE LENSEFRAFS SFKNRVYLDT VSGSLTIYNL TSSDEDEYEM ESPNITDTMK FFLYVLESLP SPTLTCALTN GSIEVQCMIP EHYNSHRGLI MYSWDCPMEQ CKRNSTSIYF KMENDLPQKI QCTLSNPLFN TTSSIILTTC IPSSGHSRHR HHHHHH. It is sometimes possible for the material contained within the vial of "CD58, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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