Western Blot (WB)
(Western blot analysis of LFA3 expression in HELA whole cell lysates (lane 1) and K562 whole cell lysates (lane 2). LFA3 at 35KD was detected using rabbit anti- LFA3 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.)
Rabbit LFA3 Polyclonal Antibody | anti-CD58 antibody
Anti-LFA3 Antibody
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Human
Tested Species:In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Western Blot (WB)
(Western blot analysis of LFA3 expression in HELA whole cell lysates (lane 1) and K562 whole cell lysates (lane 2). LFA3 at 35KD was detected using rabbit anti- LFA3 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.)
Western Blot (WB)
(Western blot analysis of LFA3 expression in HELA whole cell lysates (lane 1) and K562 whole cell lysates (lane 2). LFA3 at 35KD was detected using rabbit anti- LFA3 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.)
Immunohistochemistry (IHC)
(LFA3 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- LFA3 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.)
Immunohistochemistry (IHC)
(LFA3 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- LFA3 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.)
Testing Data
Testing Data
Background: CD58, or lymphocyte function-associated antigen 3 (LFA-3), is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. It binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. This adhesion occurs as part of the transitory initial encounters between T cells and Antigen Presenting Cells before T cell activation, when T cells are roaming the lymph nodes looking at the surface of APCs for peptide:MHC complexes the T-cell receptors are reactive to. The LFA3 gene is mapped to chromosome 1p13, which is the same location as CD2.
2. Sewell, W. A., Palmer, R. W., Spurr, N. K., Sheer, D., Brown, M. H., Bell, Y., Crumpton, M. J. The human LFA-3 gene is located at the same chromosome band as the gene for its receptor CD2. Immunogenetics 28: 278-282, 1988.
3. Wang, J., Smolyar, A., Tan, K., Liu, J., Kim, M., Sun, Z. J., Wagner, G., Reinherz, E. L. Structure of a heterophilic adhesion complex between the human CD2 and CD58 (LFA-3) counterreceptors. Cell 97: 791-803, 1999.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]
Uniprot Description
CD58: Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen- presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, GPI anchor; Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1p13
Cellular Component: cell surface; membrane; plasma membrane
Molecular Function: protein binding; receptor binding
Biological Process: heterotypic cell-cell adhesion; leukocyte migration
Research Articles on CD58
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Product Notes
The CD58 cd58 (Catalog #AAA178408) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-LFA3 Antibody reacts with Human No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's LFA3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot: 0.1-0.5mug/ml; Tested Species: Human Immunohistochemisry Paraffin: 0.5-1mug/ml; Tested Species: Human Tested Species:In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Researchers should empirically determine the suitability of the CD58 cd58 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LFA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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