Rabbit NOD1 Polyclonal Antibody | anti-NOD1 antibody

NOD1 Polyclonal Antibody

Cat. Num. AAA128101

• Gene Names: NOD1; CARD4; NLRC1; CLR7.1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: NOD1; Polyclonal Antibody; NOD1 Polyclonal Antibody; CARD4; CLR7.1; NLRC1; anti-NOD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 953

• Applicable Applications for anti-NOD1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:100
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human NOD1
• Calculated Molecular Weight: 108kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of mouse lung, using NOD1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Related Product Information for anti-NOD1 antibody

Nod1/CARD4 is a cytosolic protein structually related to Apaf-1 and plant drug-resistance proteins that has been implicated in apoptosis and inflammatory responses to certain pathogenic bacteria (1-3). It contains an amino-terminal caspase recruitment domain (CARD) that is linked to a central nucleotide-binding domain (NBD; also known as a NOD domain) and is followed by carboxy-terminal leucine-rich repeats (LRR) (1). Like Apaf-1, Nod1 induces apoptosis by a CARD/NBD-dependent activation of caspase-9 (1). The primary function of Nod1 is thought to be as a sensor for certain pathogenic microbes and triggering inflammatory responses including the activation of NF-kappaB and JNK pathways (4-6). The LRR of Nod1 appears to be involved in recognition of microbial components and the CARD domain induces NF-kappaB activation in cooperation with the CARD containing kinase, RICK/RIP2/CARDIAK (1,5,6). Mutations in Nod1 have been linked increased susceptibility to asthma (7) and inflammatory bowel disease (8).

Product Categories/Family for anti-NOD1 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 20137579
• NCBI GeneID: 10392
• UniProt Accession #: Q9Y239; Q549U4; Q8IWF5; B4DTU3
• Molecular Weight: 953
• NCBI Official Full Name: Nucleotide-binding oligomerization domain-containing protein 1
• NCBI Official Synonym Full Names: nucleotide-binding oligomerization domain containing 1
• NCBI Official Symbol: NOD1
• NCBI Official Synonym Symbols: CARD4; NLRC1; CLR7.1
• NCBI Protein Information: nucleotide-binding oligomerization domain-containing protein 1; NLR family, CARD domain containing 1; caspase recruitment domain family, member 4; caspase recruitment domain-containing protein 4; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 1
• UniProt Protein Name: Nucleotide-binding oligomerization domain-containing protein 1
• Protein Family: Nucleotide-binding oligomerization domain-containing protein
• UniProt Gene Name: NOD1
• UniProt Synonym Gene Names: CARD4
• UniProt Entry Name: NOD1_HUMAN

NCBI Description

This gene encodes a member of the NOD (nucleotide-binding oligomerization domain) family. This member is a cytosolic protein. It contains an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and 10 tandem leucine-rich repeats (LRRs) in its C terminus. The CARD is involved in apoptotic signaling, LRRs participate in protein-protein interactions, and mutations in the NBD may affect the process of oligomerization and subsequent function of the LRR domain. This protein is an intracellular pattern-recognition receptor (PRR) that initiates inflammation in response to a subset of bacteria through the detection of bacterial diaminopimelic acid. Multiple alternatively spliced transcript variants differring in the 5' UTR have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Oct 2009]

Uniprot Description

NOD1: Enhances caspase-9-mediated apoptosis. Induces NF-kappa- B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. Homodimer. Self-associates. Binds to caspase-9 and RIPK2 by CARD-CARD interaction. Interacts with ARHGEF2. Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon.

Chromosomal Location of Human Ortholog: 7p14.3

Cellular Component: basolateral plasma membrane; cytoplasm; apical plasma membrane; cytosol

Molecular Function: identical protein binding; protein binding; peptidoglycan binding; protein homodimerization activity; CARD domain binding; caspase activator activity; ATP binding

Biological Process: detection of bacterium; apoptosis; activation of MAPK activity; positive regulation of dendritic cell antigen processing and presentation; stress-activated MAPK cascade; positive regulation of JNK cascade; positive regulation of caspase activity; defense response; toll-like receptor 3 signaling pathway; signal transduction; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; interleukin-8 biosynthetic process; JNK cascade; detection of biotic stimulus; inflammatory response; toll-like receptor 4 signaling pathway; caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; protein oligomerization; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; defense response to bacterium; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway