Rabbit anti-Human Cdk2 Polyclonal Antibody | anti-Cdk2 antibody

Cdk2, Phosphorylated (Thr160) (Cell Division Protein Kinase 2, p33 Protein Kinase) (MaxLight 750)

Cat. Num. AAA6468498

• Gene Names: CDK2; CDKN2; p33(CDK2)
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Cdk2; Polyclonal Antibody; Phosphorylated (Thr160) (Cell Division Protein Kinase 2; p33 Protein Kinase) (MaxLight 750); anti-Cdk2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Cdk2 phosphorylated at Thr160.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-Cdk2 antibody: FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic phosphopeptide mapping to a fragment of residues surrounding Thr160 of human Cdk2, Swiss/UniProt Accession: P24941. Species sequence homology: bovine, hamster, mouse, rat
• Conjugate: MaxLight750
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot detecting Phospho-Cdk2-T160 in A2058 tissue lysate usingMBS629860.)

Related Product Information for anti-Cdk2 antibody

Cdk2 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle.

Product Categories/Family for anti-Cdk2 antibody

Antibodies; Protein Kinases

References

1. Bicaku, E., et al., Tissue Cell 37(1):53-58 (2005). 2. Lolli, G., et al., Structure (Camb.) 12(11):2067-2079 (2004). 3. Ito, S., et al., Genes Cells 9(10):983-992 (2004). 4. Zhou, M., et al., Proc. Natl. Acad. Sci. USA 100(22):12666-12671 (2003). 5. Kino, T., et al., Biochem. Biophys. Res. Commun. 298(1):17-23 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 16936528
• NCBI GeneID: 1017
• NCBI Accession #: NP_001789
• NCBI GenBank Nucleotide #: NM_001798.4
• UniProt Accession #: P24941; O75100; A8K7C6
• Molecular Weight: 35.0 kDa (306aa), confirmed by MALDI-TOF
• NCBI Official Full Name: cyclin-dependent kinase 2 isoform 1
• NCBI Official Synonym Full Names: cyclin dependent kinase 2
• NCBI Official Symbol: CDK2
• NCBI Official Synonym Symbols: CDKN2; p33(CDK2)
• NCBI Protein Information: cyclin-dependent kinase 2
• UniProt Protein Name: Cyclin-dependent kinase 2
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK2
• UniProt Synonym Gene Names: CDKN2
• UniProt Entry Name: CDK2_HUMAN

NCBI Description

This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

CDK2: a protein kinase of the CDK family. An important component of the cell cycle machinery. Activity of CDK2 is maximal during S phase and G2. Cdk2/cyclin E kinase activity is important for the G1 to S transition and phosphorylates the Rb protein. In S-phase, active cdk2/cyclin A complexes predominate and phosphorylate E2F, and the active cdk2 complex persists in the nucleus through G2. Part of the Rb pathway disregulated in most tumors. Target of several candidate cancer drugs. However, inhibition does not always prevent cancer cell growth, possibly due to CDK redundancy. Inhibitors: BMS-265246, BMS-265246-01, R-roscovitine (CYC200, CYC202), SU9516, R547, L868276

Protein type: Protein kinase, CMGC; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; Kinase, protein; CMGC group; CDK family; CDK1 subfamily; CDK/CDK1 subfamily

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; Y chromosome; Cajal body; centrosome; chromosome, telomeric region; transcription factor complex; X chromosome; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; condensed chromosome; nucleus; cytosol; endosome

Molecular Function: cyclin binding; protein binding; cyclin-dependent protein kinase activity; metal ion binding; histone kinase activity; ATP binding

Biological Process: G1 DNA damage checkpoint; mitosis; meiosis; histone phosphorylation; positive regulation of transcription, DNA-dependent; DNA repair; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; peptidyl-serine phosphorylation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; Ras protein signal transduction; positive regulation of cell proliferation; mitotic cell cycle; blood coagulation; DNA replication; G2/M transition of mitotic cell cycle; centrosome duplication; potassium ion transport; positive regulation of DNA replication initiation; G1/S transition of mitotic cell cycle