Western Blot (WB)
(Western blot analysis of NF-H expression in rat brain (A) and mouse brain (B) tissue extracts.)
Mouse NF-H Monoclonal Antibody | anti-NF-H antibody
NF-H Antibody
IHC: 1:50-1:500
IP: 1-2ug per 100-500ug of total protein (1 ml of cell lysate)
Western Blot (WB)
(Western blot analysis of NF-H expression in rat brain (A) and mouse brain (B) tissue extracts.)
Western Blot (WB)
(Western blot analysis of NF-H expression in rat brain (A) and mouse brain (B) tissue extracts.)
Testing Data
(Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing membrane and cytoplasmic staining of Purkinje cells and neuropil staining in granular layer and molecular layer (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing membrane and cytoplasmic staining of Purkinje cells and neuropil staining in granular layer and molecular layer (B).)
Testing Data
(Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing membrane and cytoplasmic staining of Purkinje cells and neuropil staining in granular layer and molecular layer (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing membrane and cytoplasmic staining of Purkinje cells and neuropil staining in granular layer and molecular layer (B).)
NCBI and Uniprot Product Information
NCBI Description
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]
Uniprot Description
NFH: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. Belongs to the intermediate filament family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 22q12.2
Cellular Component: mitochondrion; axon; cytoplasm; neurofilament
Molecular Function: protein binding, bridging; dynein binding; structural constituent of cytoskeleton; kinesin binding; microtubule binding; structural molecule activity; protein kinase binding
Biological Process: axonogenesis; neurofilament bundle assembly; microtubule cytoskeleton organization and biogenesis; cell projection biogenesis; peripheral nervous system neuron axonogenesis
Disease: Amyotrophic Lateral Sclerosis 1
Research Articles on NF-H
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Product Notes
The NF-H nefh (Catalog #AAA9435181) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NF-H Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NF-H can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin. WB: 1:500-1:1000 IHC: 1:50-1:500 IP: 1-2ug per 100-500ug of total protein (1 ml of cell lysate). Researchers should empirically determine the suitability of the NF-H nefh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NF-H, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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