Rabbit anti-Mouse PD-L1 Monoclonal Antibody | anti-CD274 antibody

Recombinant Anti-PD-L1 Antibody (APC), Rabbit Monoclonal

Cat. Num. AAA8110757

• Gene Names: Cd274; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: PD-L1; Monoclonal Antibody; Recombinant Anti-PD-L1 Antibody (APC); Rabbit Monoclonal; PD-L1/B7-H1/CD274 Antibody (APC); Rabbit MAb; CD274 molecule; Anti-A530045L16Rik Antibody; Anti-B7h1 Antibody; Anti-Pdcd1l1 Antibody; Anti-Pdcd1lg1 Antibody; Anti-Pdl1 Antibody; anti-CD274 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 485
• Specificity: Mouse PD-L1/B7-H1/CD274
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 5ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-CD274 antibody: Flow Cytometry (FC/FACS/FCM)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Mouse PD-L1/B7-H1/CD274 protein
• Conjugation: APC
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse PD-L1/B7-H1/CD274 (rM PD-L1/B7-H1/CD274) and conjugated with APC under optimum conditions, the unreacted APC was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Testing Data

(Analysis of CD274 expression on spleen lymphocytes.)

Related Product Information for anti-CD274 antibody

Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

Product Categories/Family for anti-CD274 antibody

Cancer Drug Targets

References

Iwai Y, et al. (2002) Involvement of PD-L1 on tumor cells in the escape from host immune system and tumor immunotherapy by PD-L1 blockade. Proc Natl Acad Sci U S A. 99(19): 12293-7.Ghebeh H, et al. (2006) The B7-H1 (PD-L1) T lymphocyte-inhibitory molecule is expressed in breast cancer patients with infiltrating ductal carcinoma: correlation with important high-risk prognostic factors. Neoplasia. 8(3): 190-8.Salih HR, et al. (2006) The role of leukemia-derived B7-H1 (PD-L1) in tumor-T-cell interactions in humans. Exp Hematol. 34(7): 888-94.Wilcox RA, et al. (2009) B7-H1 (PD-L1, CD274) suppresses host immunity in T-cell lymphoproliferative disorders. Blood. 114(10): 2149-58.Ruggiero A, et al. (2009) Crystal structure of PD-L1, a ribosome inactivating protein from Phytolacca dioica L. leaves with the property to induce DNA cleavage. Biopolymers. 91(12): 1135-42.

NCBI and Uniprot Product Information

• NCBI GI #: 11230798
• NCBI GeneID: 60533
• NCBI Accession #: NP_068693.1
• NCBI GenBank Nucleotide #: NM_021893.3
• UniProt Accession #: Q9EP73
• NCBI Official Full Name: programmed cell death 1 ligand 1
• NCBI Official Synonym Full Names: CD274 antigen
• NCBI Official Symbol: Cd274
• NCBI Official Synonym Symbols: B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
• NCBI Protein Information: programmed cell death 1 ligand 1; B7 homolog 1; PDCD1 ligand 1; programmed death ligand 1
• UniProt Protein Name: Programmed cell death 1 ligand 1
• Protein Family: Programmed cell death
• UniProt Gene Name: Cd274
• UniProt Synonym Gene Names: B7h1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
• UniProt Entry Name: PD1L1_MOUSE

Uniprot Description

CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding

Biological Process: cell surface receptor linked signal transduction; negative regulation of T cell proliferation; negative regulation of interleukin-10 production; negative regulation of activated T cell proliferation; negative regulation of interferon-gamma production; T cell costimulation; positive regulation of T cell proliferation; immune response; signal transduction; positive regulation of cell migration