Cluster Of Differentiation 14 (CD14) Recombinant Protein | CD14 recombinant protein

Recombinant Cluster Of Differentiation 14 (CD14)

Cat. Num. AAA2012044

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cluster Of Differentiation 14 (CD14); Recombinant Cluster Of Differentiation 14 (CD14); CD14 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-SPA TPEPCELDQD EESVRCYCNF SDPQPNWSSA FLCAGAEDVE FYGGGRSLEY LLKRVDTEAN LGQYTDIIRS LPLKRLTVRS ARVPTQILFG TLRVLGYSGL RELTLENLEV TGTALSPLLD ATGPDLNTLS LRNVSWATTD TWLAELQQWL KPGLKVLSIA QAHSLNFSCK QVGVFPALAT LDLSDNPELG EKGLISALCP HKFPTLQVLA LRNAGMETTS GVCSALAAAR VPLQALDLSH NSLRDTAGTP SCDWPSQLNS LNLSFTGLEH VPKGLPAKLS VLDLSYNRLD RKPRPEELPE VGSLSLTGNPFLHSESQSEA YNSGVVIATA LSPGSAGLSG TLALLLGHRL FV
• Sequence Length: 372

• Applicable Applications for CD14 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Ser18~Val372 (Accession # Q63691) with two N-terminal Tags, His-tag and GST-tag
• Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 5.7
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 11120688
• NCBI GeneID: 60350
• NCBI Accession #: NP_068512.1
• NCBI GenBank Nucleotide #: NM_021744.1
• UniProt Accession #: Q63691; Q6GT04
• Molecular Weight: 69.9kDa
• NCBI Official Full Name: monocyte differentiation antigen CD14
• NCBI Official Synonym Full Names: CD14 molecule
• NCBI Official Symbol: Cd14
• NCBI Protein Information: monocyte differentiation antigen CD14; CD14 antigen; myeloid cell-specific leucine-rich glycoprotein
• UniProt Protein Name: Monocyte differentiation antigen CD14
• Protein Family: Monocyte differentiation antigen
• UniProt Gene Name: Cd14
• UniProt Entry Name: CD14_RAT

NCBI Description

component of the lipopolysaccharide receptor complex; mediates LPS-induced neuroinflammation and inflammatory response [RGD, Feb 2006]

Uniprot Description

Function: In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules

By similarity.

Subunit structure: Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4

By similarity.

Subcellular location: Cell membrane; Lipid-anchor › GPI-anchor.

Sequence similarities: Contains 11 LRR (leucine-rich) repeats.