Cluster Of Differentiation 40 Ligand (CD40L) Recombinant Protein | CD40L recombinant protein

Recombinant Cluster Of Differentiation 40 Ligand (CD40L)

Cat. Num. AAA2011898

• Gene Names: CD40LG; TNFSF5
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cluster Of Differentiation 40 Ligand (CD40L); Recombinant Cluster Of Differentiation 40 Ligand (CD40L); CD40L recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-MHKGDQEP QIAAHVISEA SSKTTSVLQW APKGYYTLSN NLVTLENGKQ LAVKRQGFYY IYTQVTFCSN RETLSQAPFI ASLCLKSPSG SERILLRAAN THSSSKPCGQ QSIHLGGVFE LQSGASVFVN VTDPSQVSHG TGFTSFGLLK L
• Sequence Length: 261

• Applicable Applications for CD40L recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Bos taurus; Bovine (Cattle)
• Expression System: Prokaryotic expression
• Residues: Met113~Leu261 (Accession # P51749) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 27807127
• NCBI GeneID: 282387
• NCBI Accession #: NP_777049.1
• NCBI GenBank Nucleotide #: NM_174624.2
• UniProt Accession #: P51749
• Molecular Weight: 48.1kDa
• NCBI Official Full Name: CD40 ligand
• NCBI Official Synonym Full Names: CD40 ligand<
• NCBI Official Symbol: CD40LG
• NCBI Official Synonym Symbols: TNFSF5
• NCBI Protein Information: CD40 ligand; CD40-L; human : hyper-IgM syndrome; tumor necrosis factor ligand superfamily member 5; tumor necrosis factor (ligand) superfamily, member 5; CD40 ligand (TNF superfamily, member 5, hyper-IgM syndrome)
• UniProt Protein Name: CD40 ligand
• UniProt Gene Name: CD40LG
• UniProt Synonym Gene Names: CD40L; TNFSF5; CD40-L
• UniProt Entry Name: CD40L_BOVIN

Uniprot Description

Function: Cytokine that binds to TNFRSF5. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching

By similarity.Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway

By similarity.

Subunit structure: Homotrimer

By similarity.

Subcellular location: Cell membrane; Single-pass type II membrane protein

By similarity. CD40 ligand, soluble form: Secreted

By similarity.

Post-translational modification: The soluble form derives from the membrane form by proteolytic processing

By similarity.

Sequence similarities: Belongs to the tumor necrosis factor family.