Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Cluster Of Differentiation 14 (CD14) Polyclonal Antibody | anti-CD14 antibody
Polyclonal Antibody to Cluster Of Differentiation 14 (CD14)
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Cluster Of Differentiation 14 (CD14); Polyclonal Antibody; Polyclonal Antibody to Cluster Of Differentiation 14 (CD14); anti-CD14 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CD14. It has been selected for its ability to recognize CD14 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-SPA TPEPCELDQD EESVRCYCNF SDPQPNWSSA FLCAGAEDVE FYGGGRSLEY LLKRVDTEAN LGQYTDIIRS LPLKRLTVRS ARVPTQILFG TLRVLGYSGL RELTLENLEV TGTALSPLLD ATGPDLNTLS LRNVSWATTD TWLAELQQWL KPGLKVLSIA QAHSLNFSCK QVGVFPALAT LDLSDNPELG EKGLISALCP HKFPTLQVLA LRNAGMETTS GVCSALAAAR VPLQALDLSH NSLRDTAGTP SCDWPSQLNS LNLSFTGLEH VPKGLPAKLS VLDLSYNRLD RKPRPEELPE VGSLSLTGNP FLHSESQSEA YNSGVVIATA LSPGSAGLSG TLALLLGHRL FV
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-SPA TPEPCELDQD EESVRCYCNF SDPQPNWSSA FLCAGAEDVE FYGGGRSLEY LLKRVDTEAN LGQYTDIIRS LPLKRLTVRS ARVPTQILFG TLRVLGYSGL RELTLENLEV TGTALSPLLD ATGPDLNTLS LRNVSWATTD TWLAELQQWL KPGLKVLSIA QAHSLNFSCK QVGVFPALAT LDLSDNPELG EKGLISALCP HKFPTLQVLA LRNAGMETTS GVCSALAAAR VPLQALDLSH NSLRDTAGTP SCDWPSQLNS LNLSFTGLEH VPKGLPAKLS VLDLSYNRLD RKPRPEELPE VGSLSLTGNP FLHSESQSEA YNSGVVIATA LSPGSAGLSG TLALLLGHRL FV
Applicable Applications for anti-CD14 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant CD14 (Ser18~Val372) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2046021
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,054 Da
NCBI Official Full Name
monocyte differentiation antigen CD14
NCBI Official Synonym Full Names
CD14 molecule
NCBI Official Symbol
Cd14
NCBI Protein Information
monocyte differentiation antigen CD14
UniProt Protein Name
Monocyte differentiation antigen CD14
Protein Family
UniProt Gene Name
Cd14
NCBI Description
component of the lipopolysaccharide receptor complex; mediates LPS-induced neuroinflammation and inflammatory response [RGD, Feb 2006]
Uniprot Description
Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- ().
Research Articles on CD14
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Product Notes
The CD14 cd14 (Catalog #AAA2001438) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cluster Of Differentiation 14 (CD14) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cluster Of Differentiation 14 (CD14) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CD14 cd14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-S PA TPEPCELDQD EESVRCYCNF SDPQPNWSSA FLCAGAEDVE FYGGGRSLEY LLKRVDTEAN LGQYTDIIRS LPLKRLTVRS ARVPTQILFG TLRVLGYSGL RELTLENLEV TGTALSPLLD ATGPDLNTLS LRNVSWATTD TWLAELQQWL KPGLKVLSIA QAHSLNFSCK QVGVFPALAT LDLSDNPELG EKGLISALCP HKFPTLQVLA LRNAGMETTS GVCSALAAAR VPLQALDLSH NSLRDTAGTP SCDWPSQLNS LNLSFTGLEH VPKGLPAKLS VLDLSYNRLD RKPRPEELPE VGSLSLTGNP FLHSESQSEA YNSGVVIATA LSPGSAGLSG TLALLLGHRL FV. It is sometimes possible for the material contained within the vial of "Cluster Of Differentiation 14 (CD14), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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