CCL22/MDC recombinant protein

CCL22/MDC, 25-93aa, Human, His tag, E Coli

Cat. Num. AAA203398

• Gene Names: CCL22; MDC; ABCD-1; SCYA22; STCP-1; DC/B-CK; A-152E5.1
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: CCL22/MDC; 25-93aa; Human; His tag; E Coli; Chemokine (C-C motif) ligand 22 / Macrophage-Derived Chemokine; SCYA22; STCP-1; Chemokine (C-C motif) ligand 22 / Macrophage-Derived Chemokine DC/B CK; A 152E5.1; ABCD 1; ABCD1; CC chemokine STCP 1; ccl 22; CCL22; MGC34554; Small inducible cytokine subfamily A (Cys Cys) member 22; STCP 1; STCP1; Stimulated T cell chemotactic protein 1; CCL22/MDC recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate-buffered saline (PBS) pH7.4, 10% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGPYGANMED SVCCRDYVRY RLPLRVVKHF YWTSDSCPRP GVVLLTFRDK EICADPRVPW VKMILNKLSQ
• Sequence Length: 93

• Applicable Applications for CCL22/MDC recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CCL22/MDC recombinant protein

CCL22/MDC is a small cytokine belonging to the CC chemokine family. This protien displays chemotactic activity for natural killer cells, chronically activated T lymphocytes, monocytes and dendritic cells. It interacts with cell surface chemokine receptors CCR4. This chemokine may play a role in the trafficking of activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology. Recombinant human CCL22/MDC, fused to His-tag at N-terminus, was expressed as insoluble protein aggregate in E Coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Categories/Family for CCL22/MDC recombinant protein

Hormones & Cytokines

References

Wang G., et al. (2009). Cancer. 115 (11):2430-7; Silvano Sozzani., et al. (2000). Journal of Leukocyte Biology. 68:400-404;

NCBI and Uniprot Product Information

• NCBI GI #: 22538804
• NCBI GeneID: 6367
• NCBI Accession #: NP_002981
• NCBI GenBank Nucleotide #: NM_002990.4
• UniProt Accession #: O00626; A0N0Q6; B2R4W2
• Molecular Weight: 10.3 kDa (90aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: C-C motif chemokine 22
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 22
• NCBI Official Symbol: CCL22
• NCBI Official Synonym Symbols: MDC; ABCD-1; SCYA22; STCP-1; DC/B-CK; A-152E5.1
• NCBI Protein Information: C-C motif chemokine 22
• UniProt Protein Name: C-C motif chemokine 22
• UniProt Gene Name: CCL22
• UniProt Synonym Gene Names: MDC; SCYA22

NCBI Description

This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, dendritic cells, natural killer cells and for chronically activated T lymphocytes. It also displays a mild activity for primary activated T lymphocytes and has no chemoattractant activity for neutrophils, eosinophils and resting T lymphocytes. The product of this gene binds to chemokine receptor CCR4. This chemokine may play a role in the trafficking of activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology. [provided by RefSeq, Sep 2014]

Uniprot Description

May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active.