Human MDC ELISA Kit | MDC elisa kit

MDC (Human) OmniKine ELISA Kit

Cat. Num. AAA9502029

• Gene Names: CCL22; MDC; ABCD-1; SCYA22; STCP-1; DC/B-CK; A-152E5.1
• Reactivity: Human
• Synonyms: MDC; MDC (Human) OmniKine ELISA Kit; C-C motif chemokine 22; CC chemokine STCP-1; Macrophage-derived chemokine; Small-inducible cytokine A22; Stimulated T-cell chemotactic protein 1; MDC elisa kit

• Reactivity: Human
• Specificity: The OmniKine™ Human MDC ELISA is capable of recognizing both recombinant and naturally produced Human MDC proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross-reactivity or interference.
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 93

• Assay Type: Quantitative Sandwich
• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The OmniKine Human MDC ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human MDC proteins within the range of 8-500 pg/ml.)

Related Product Information for MDC elisa kit

Principle of the Assay: The OmniKine™ Human MDC ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human MDC concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human MDC while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

Background/Introduction: C-C motif chemokine 22 (CCL22), also known as MDC, is a 93 amino acid secreted protein that is highly expressed in macrophage, monocyte-derived dendritic cells, and thymus. It is also found in the lymph node, appendix, activated monocytes, resting and activated macrophages. Lower expression of CCL22 is found in the lung and spleen, and very weak expression is found in the small intestine. It is also expressed in a mature subset of Langerhans' cells (CD1a+ and CD83+) in the lymph node as well as in Langerhans' cell histiocytosis but not in dermatopathic lymphadenopathy. Expression of CCL22 is also found in atopic dermatitis, allergic contact dermatitis skin, and psoriasis, in both the epidermis and dermis. CCL22 may play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. It is a chemotactic factor for monocytes, dendritic cells and natural killer cells; it is a mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. CCL22 Binds to CCR4. Its processed forms MDC (3-69), MDC (5-69) and MDC (7-69) seem not be active. The N-terminal processed forms MDC (3-69), MDC (5-69) and MDC (7-69) are produced by proteolytic cleavage after secretion from monocyte derived dendrocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 22538804
• NCBI GeneID: 6367
• NCBI Accession #: NP_002981.2
• NCBI GenBank Nucleotide #: NM_002990.4
• UniProt Accession #: O00626; A0N0Q6; B2R4W2
• Molecular Weight: 10,625 Da
• NCBI Official Full Name: C-C motif chemokine 22
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 22
• NCBI Official Symbol: CCL22
• NCBI Official Synonym Symbols: MDC; ABCD-1; SCYA22; STCP-1; DC/B-CK; A-152E5.1
• NCBI Protein Information: C-C motif chemokine 22
• UniProt Protein Name: C-C motif chemokine 22
• UniProt Gene Name: CCL22
• UniProt Synonym Gene Names: MDC; SCYA22

NCBI Description

This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, dendritic cells, natural killer cells and for chronically activated T lymphocytes. It also displays a mild activity for primary activated T lymphocytes and has no chemoattractant activity for neutrophils, eosinophils and resting T lymphocytes. The product of this gene binds to chemokine receptor CCR4. This chemokine may play a role in the trafficking of activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL22: May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: extracellular region; extracellular space

Molecular Function: CCR chemokine receptor binding; chemokine activity

Biological Process: cell-cell signaling; chemotaxis; G-protein coupled receptor signaling pathway; immune response; inflammatory response; lymphocyte chemotaxis; monocyte chemotaxis; neutrophil chemotaxis; positive regulation of GTPase activity; response to virus; signal transduction