Typical Testing Data/Standard Curve (for reference only)
Mouse Semaphorin-4D, SEMA4D ELISA Kit | SEMA4D elisa kit
Mouse Semaphorin-4D, SEMA4D ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse SEMA4D antibody. SEMA4D present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SEMA4D Antibody is added and binds to SEMA4D in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SEMA4D antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse SEMA4D. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
Uniprot Description
SEMA4D: a single-pass type I membrane protein that my play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 9q22.2
Cellular Component: extracellular space; integral to plasma membrane; plasma membrane
Molecular Function: protein binding; transmembrane receptor activity; receptor activity; semaphorin receptor binding; receptor binding
Biological Process: axon guidance; negative regulation of peptidyl-tyrosine phosphorylation; regulation of dendrite morphogenesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell adhesion; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell shape; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of osteoblast differentiation; immune response; positive regulation of protein amino acid phosphorylation; cell adhesion; positive regulation of collateral sprouting; regulation of cell projection organization and biogenesis; negative regulation of apoptosis; positive regulation of cell migration
Research Articles on SEMA4D
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Product Notes
The Mouse SEMA4D sema4d (Catalog #AAA1605651) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1605651 ELISA Kit recognizes Mouse SEMA4D. It is sometimes possible for the material contained within the vial of "Semaphorin-4D, SEMA4D, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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