MDC Recombinant Protein | CCL22 recombinant protein

MDC Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120041

• Gene Names: CCL22; MDC; ABCD-1; SCYA22; STCP-1; DC/B-CK; A-152E5.1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: MDC; MDC Protein; Human; Recombinant (His Tag); Human CCL22/MDC Protein (His Tag); A-152E5.1 Protein; ABCD-1 Protein; DC/B-CK Protein; SCYA22 Protein; STCP-1 Protein; chemokine (C-C motif) ligand 22; CCL22 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 30% Acetonitrile, 0.1% TFA. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gly25-Gln93

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the secreeted form of human CCL22 (O00626)(Gly 25-Gln 93) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CCL22 recombinant protein

Background: Chemokine (C-C motif) ligand 22(ABCD-1/CCL22)is a kind of CC chemokine which is a family of secreted proteins involved in immunoregulatory and inflammatory processes. The cytokine displays chemotactic activity for monocytes, dendritic cells, natural killer cells and for chronically activated T lymphocytes. It also displays a mild activity for primary activated T lymphocytes and has no chemoattractant activity for neutrophils, eosinophils and resting T lymphocytes. This ABCD-1/CCL22 chemokine binds to chemokine receptor CCR4. This chemokine may play a role in the trafficking of activated/effector T lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. ABCD-1/CCL22 is highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus, and in Langerhans' cell histiocytosis and atopic dermatitis but not in dermatopathic lymphadenopathy. This chemokine is also found in lymph node, appendix, activated monocytes, resting and activated macrophages. This protein is lower expressed in lung and spleen and very weekly expressed in small intestine.

References

Vulcano M, et al. (2001) Dendritic cells as a major source of macrophage-derived chemokine/CCL22 in vitro and in vivo. Eur J Immunol. 31(3): 812-22.Kwon DJ, et al. (2011) Casuarinin suppresses TARC/CCL17 and MDC/CCL22 production via blockade of NF-B and STAT1 activation in HaCaT cells. Biochem Biophys Res Commun. 417(4):1254-9.Hirota T, et al. (2011) Variants of C-C motif chemokine 22 (CCL22) are associated with susceptibility to atopic dermatitis: case-control studies. PLoS One. 6(11):e26987.

NCBI and Uniprot Product Information

• NCBI GI #: 1931581
• NCBI GeneID: 6367
• UniProt Accession #: O00626; A0N0Q6; B2R4W2
• Molecular Weight: 10,625 Da
• NCBI Official Full Name: macrophage-derived chemokine
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 22
• NCBI Official Symbol: CCL22
• NCBI Official Synonym Symbols: MDC; ABCD-1; SCYA22; STCP-1; DC/B-CK; A-152E5.1
• NCBI Protein Information: C-C motif chemokine 22; MDC(1-69); CC chemokine STCP-1; macrophage-derived chemokine; small inducible cytokine A22; small-inducible cytokine A22; stimulated T cell chemotactic protein 1; stimulated T-cell chemotactic protein 1; small inducible cytokine su
• UniProt Protein Name: C-C motif chemokine 22
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL22
• UniProt Synonym Gene Names: MDC; SCYA22
• UniProt Entry Name: CCL22_HUMAN

NCBI Description

This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, dendritic cells, natural killer cells and for chronically activated T lymphocytes. It also displays a mild activity for primary activated T lymphocytes and has no chemoattractant activity for neutrophils, eosinophils and resting T lymphocytes. The product of this gene binds to chemokine receptor CCR4. This chemokine may play a role in the trafficking of activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology. [provided by RefSeq, Jul 2008]

Uniprot Description

CCL22: May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Chemokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity

Biological Process: cell-cell signaling; response to virus; immune response; signal transduction; inflammatory response; chemotaxis