Mouse chemokine (C-C motif) ligand 22 ELISA Kit | CCL22 elisa kit

Mouse Macrophage-Derived Chemokine, MDC ELISA Kit

Cat. Num. AAA702047

• Gene Names: Ccl22; MDC; DCBCK; ABCD-1; Scya22
• Reactivity: Mouse
• Synonyms: chemokine (C-C motif) ligand 22; Mouse Macrophage-Derived Chemokine; MDC ELISA Kit; Mouse Macrophage-Derived Chemokine (MDC/CCL22) ELISA kit; A-152E5.1; ABCD-1; DC/B-CK; MDC; MGC34554; SCYA22; STCP-1; CC chemokine STCP-1; macrophage-derived chemokine; small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys); member 22; sti; Mouse Macrophage-Derived Chemokine (MDC/CCL22); CCL22 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse MDC. No significant cross-reactivity or interference between mouse MDC and analogues was observed.
• Sequence Length: 92

• Samples: Serum, plasma, tissue homogenates, cell culture supernates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 31.25 pg/ml -2000 pg/ml.
• Sensitivity: The minimum detectable dose of mouse MDC is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL22 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MDC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MDC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDC bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6677879
• NCBI GeneID: 20299
• NCBI Accession #: NP_033163.1
• NCBI GenBank Nucleotide #: NM_009137.2
• UniProt Accession #: O88430
• Molecular Weight: 10,302 Da
• NCBI Official Full Name: C-C motif chemokine 22
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 22
• NCBI Official Symbol: Ccl22
• NCBI Official Synonym Symbols: MDC; DCBCK; ABCD-1; Scya22
• NCBI Protein Information: C-C motif chemokine 22; CC chemokine ABCD-1; small-inducible cytokine A22; activated B and dendritic cell-derived; small inducible cytokine subfamily A22; dendritic cell and B cell derived chemokine; small inducible cytokine subfamily A, member 22; SMALL INDUCIBLE CYTOKINE A22 PRECURSOR (CC CHEMOKINE ABCD-1) (ACTIVATED B AND DENDRITIC CELL-DERIVED)
• UniProt Protein Name: C-C motif chemokine 22
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl22
• UniProt Synonym Gene Names: Abcd1; Scya22
• UniProt Entry Name: CCL22_MOUSE

Uniprot Description

Function: Chemotactic for activated T-lymphocytes. May play an important role in the collaboration of dendritic cells and B-lymphocytes with T-cells in immune responses.