Western Blot (WB)
(Western blot analysis of mouse CCL22 using recombinant mouse CCL22 diluted between 1.5 - 3.0ng/lane. Detection was performed using Rabbit anti Mouse CCL22 antibody (MBS226089) followed by a compatible secondary antibody.)
Rabbit anti-Mouse CCL22 Polyclonal Antibody | anti-CCL22 antibody
Rabbit anti Mouse CCL22:Preservative Free
Gene Names
Ccl22; MDC; DCBCK; ABCD-1; Scya22
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Produced from sera of rabbits immunized with recombinant mouse CCL22 and purified by affinity chromatography
Synonyms
CCL22; Polyclonal Antibody; Rabbit anti Mouse CCL22:Preservative Free; CCL22 antibody; anti-CCL22 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Produced from sera of rabbits immunized with recombinant mouse CCL22 and purified by affinity chromatography
Form/Format
Phosphate buffered saline
Purified IgG - lyophilized
Purified IgG - lyophilized
Concentration
IgG concentration 1.0mg/ml after reconstitution (varies by lot)
Applicable Applications for anti-CCL22 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: 0.5ug/ml to 2.0ug/ml
WB: 0.1ug/ml to 0.2ug/ml
WB: 0.1ug/ml to 0.2ug/ml
Immunogen
E Coli derived recombinant mouse CCL22
Endotoxin Level
<0.1ng/ug of protein (<1EU/ug)
Conjugation
Preservative Free
Reconstitution
Reconstitute with 0.1ml distilled water
Preparation and Storage
Prior to reconstitution store at -20 degree C.
After reconstitution store at -20 degree C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20 degree C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Western Blot (WB)
(Western blot analysis of mouse CCL22 using recombinant mouse CCL22 diluted between 1.5 - 3.0ng/lane. Detection was performed using Rabbit anti Mouse CCL22 antibody (MBS226089) followed by a compatible secondary antibody.)
Western Blot (WB)
(Western blot analysis of mouse CCL22 using recombinant mouse CCL22 diluted between 1.5 - 3.0ng/lane. Detection was performed using Rabbit anti Mouse CCL22 antibody (MBS226089) followed by a compatible secondary antibody.)
Related Product Information for anti-CCL22 antibody
Rabbit anti Mouse CCL22 antibody recognizes C-C motif chemokine 22 (CCL22), also known as macrophage-derived chemokine, small inducible cytokine A22 and stimulated T cell chemotactic protein 1.
CCL22 is a potent chemoattractant for antigen experienced T lymphocytes, natural killer cells, monocytes and dendritic cells. CCL22 elicits its biological function through its receptor CCR4 (Scheu et al. 2017).
CCL22 is a potent chemoattractant for antigen experienced T lymphocytes, natural killer cells, monocytes and dendritic cells. CCL22 elicits its biological function through its receptor CCR4 (Scheu et al. 2017).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,302 Da
NCBI Official Full Name
C-C motif chemokine 22
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 22
NCBI Official Symbol
Ccl22
NCBI Official Synonym Symbols
MDC; DCBCK; ABCD-1; Scya22
NCBI Protein Information
C-C motif chemokine 22; CC chemokine ABCD-1; SMALL INDUCIBLE CYTOKINE A22 PRECURSOR (CC CHEMOKINE ABCD-1) (ACTIVATED B AND DENDRITIC CELL-DERIVED); activated B and dendritic cell-derived; dendritic cell and B cell derived chemokine; small inducible cytoki
UniProt Protein Name
C-C motif chemokine 22
Protein Family
UniProt Gene Name
Ccl22
UniProt Synonym Gene Names
Abcd1; Scya22
UniProt Entry Name
CCL22_MOUSE
Uniprot Description
CCL22: May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Secreted; Motility/polarity/chemotaxis; Chemokine; Secreted, signal peptide
Cellular Component: extracellular space; extracellular region
Molecular Function: chemokine activity; cytokine activity
Biological Process: immune response; inflammatory response; chemotaxis
Research Articles on CCL22
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Product Notes
The CCL22 ccl22 (Catalog #AAA226089) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Mouse CCL22:Preservative Free reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CCL22 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: 0.5ug/ml to 2.0ug/ml WB: 0.1ug/ml to 0.2ug/ml. Researchers should empirically determine the suitability of the CCL22 ccl22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCL22, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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