Mouse MDC ELISA Kit | CCL22 elisa kit

Mouse MDC PicoKine ELISA Kit

Cat. Num. AAA175846

• Gene Names: Ccl22; MDC; DCBCK; ABCD-1; Scya22
• Reactivity: Mouse
• Synonyms: MDC; Mouse MDC PicoKine ELISA Kit; C-C motif chemokine 22; chemokine (C-C motif) ligand 22; A-152E5.1; ABCD 1; ABCD1; CC chemokine STCP-1; Ccl22; CCL22_HUMAN; Chemokine (C-C motif) ligand 22; DC/B-CK; Macrophage-derived chemokine; MDC(1-69); MDC(7-69); MGC34554; SCYA22; Small inducible cytokine A22; Small inducible cytokine subfamily A (Cys-Cys); member 22; Small-inducible cytokine A22; STCP 1; STCP1; Stimulated T cell chemotactic protein 1; Stimulated T-cell chemotactic protein 1; CCL22 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse MDC
• Sequence Length: 92

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: < 1pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse MDC PicoKine ELISA Kit standard curve )

Related Product Information for CCL22 elisa kit

Principle of the assay: mouse MDC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MDC has been precoated onto 96-well plates. Standards (E.coli, G25-S92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MDC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MDC amount of sample captured in plate.
Background: Macrophage-derived chemokine (MDC), also called Chemokine, cc motif, ligand 22 (CCL22) or Small inducible cytokine subfamily A, member 22 (SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine (TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares <35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 6094388
• NCBI GeneID: 20299
• UniProt Accession #: O88430
• Molecular Weight: 10,302 Da
• NCBI Official Full Name: C-C motif chemokine 22
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 22
• NCBI Official Symbol: Ccl22
• NCBI Official Synonym Symbols: MDC; DCBCK; ABCD-1; Scya22
• NCBI Protein Information: C-C motif chemokine 22; CC chemokine ABCD-1; small-inducible cytokine A22; activated B and dendritic cell-derived; small inducible cytokine subfamily A22; dendritic cell and B cell derived chemokine; small inducible cytokine subfamily A, member 22; SMALL INDUCIBLE CYTOKINE A22 PRECURSOR (CC CHEMOKINE ABCD-1) (ACTIVATED B AND DENDRITIC CELL-DERIVED)
• UniProt Protein Name: C-C motif chemokine 22
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl22
• UniProt Synonym Gene Names: Abcd1; Scya22
• UniProt Entry Name: CCL22_MOUSE

Uniprot Description

CCL22: May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted; Chemokine

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; cytokine activity

Biological Process: immune response; chemotaxis; inflammatory response