Eotaxin Recombinant Protein | Eotaxin-1 recombinant protein

Mouse Eotaxin Recombinant

Cat. Num. AAA553218

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Eotaxin; Mouse Eotaxin Recombinant; eotaxin; ccl11; scya11; Eotaxin-1 recombinant protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant mouse Eotaxin was lyophilized from a 0.2 um filtered PBS solution.
• Sequence: MQSSTALLFL LLTVTSFTSQ VLAHPGSIPT SCCFIMTSKK IPNTLLKSYK RITNNRCTLK AIVFKTRLGK EICADPKKKW VQDATKHLDQ KLQTPKP
• Sequence Length: 97

• Domain: IL8
• Host Note: Optimized DNA sequence encoding Mouse Eotaxin mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Mouse Eotaxin has a calculated molecular mass of approximately 8 kDa.; ; Recombinant mouse Eotaxin is a disulfide-linked homodimeric protein consisting 75 amino acid residue subunits, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for Eotaxin-1 recombinant protein

Eotaxin belongs to the platelet factor-4 family of chemokines. The gene symbol is SCYA11. The factor is being referred to also as Eotaxin-1 and has been renamed CCL11. Eotaxin induces substantial accumulation of eosinophils in the skin without significantly affecting the accumulation of neutrophils. Eotaxin is a potent stimulator of both guinea pig and human eosinophils in vitro. The Eotaxin receptor is a G-protein-coupled receptor selectively expressed in human eosinophils.

Product Categories/Family for Eotaxin-1 recombinant protein

Cytokines

References

• role of Eotaxin-1 signaling in ovarian cancer .Clin. Cancer Res., Apr 2009; 15: 2647 - 2656.
• ablation of type i hypersensitivity in experimental allergic conjunctivitis by Eotaxin-1/ccr3 blockade .Int. Immunol., Feb 2009; 21: 187 - 201.
• intestinal macrophage/epithelial cell-derived ccl11/Eotaxin-1 mediates eosinophil recruitment and function in pediatric ulcerative colitis .J. Immunol., Nov 2008; 181: 7390 - 7399.
• pulmonary eosinophilia requires interleukin-5, Eotaxin-1, and cd4+ t cells in mice immunized with respiratory syncytial virus g glycoprotein .J. Leukoc. Biol., Sep 2008; 84: 748 - 759.
• involvement of nfkb and stat6 on Eotaxin-1, il-8 and rantes production in human conjunctival fibroblasts and epithelial cells .Invest. Ophthalmol. Vis. Sci., Apr 2008; 49: 430.