Rabbit anti-Human Carbonic Anhydrase IX Polyclonal Antibody | anti-CA9 antibody

Anti-Carbonic Anhydrase IX Antibody, Rabbit Polyclonal

Cat. Num. AAA8100959

• Gene Names: CA9; MN; CAIX
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: Carbonic Anhydrase IX; Polyclonal Antibody; Anti-Carbonic Anhydrase IX Antibody; Rabbit Polyclonal; Carbonic Anhydrase IX/CA9 Antibody; Rabbit PAb; Antigen Affinity Purified; carbonic anhydrase IX; Anti-CAIX Antibody; Anti-MN Antibody; anti-CA9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Carbonic Anhydrase IX/CA9
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CA9 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000
• Immunogen: Recombinant Human Carbonic Anhydrase IX/CA9 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase IX/CA9 (rh Carbonic Anhydrase IX/CA9). Carbonic Anhydrase IX/CA9 specific IgG was purified by Human Carbonic Anhydrase IX/CA9 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-CAIX rabbit polyclonal antibody at 1:500 dilutionLane A: HT-29 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:50 kDaObserved band size:63 kDa)

Related Product Information for anti-CA9 antibody

Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3-) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.

Product Categories/Family for anti-CA9 antibody

Cancer Drug Targets

References

Loncaster JA, et al. (2001) Carbonic anhydrase (CA IX) expression, a potential new intrinsic marker of hypoxia: correlations with tumor oxygen measurements and prognosis in locally advanced carcinoma of the cervix. Cancer Res. 61(17): 6394-9.
Zvada J, et al. (2003) Soluble form of carbonic anhydrase IX (CA IX) in the serum and urine of renal carcinoma patients. Br J Cancer. 89(6): 1067-71.
Pan P, et al. (2006) Carbonic anhydrase gene expression in CA II-deficient (Car2-/-) and CA IX-deficient (Car9-/-) mice. J Physiol. 571(Pt 2): 319-27.
Zhou GX, et al. (2010) Quantification of carbonic anhydrase IX expression in serum and tissue of renal cell carcinoma patients using enzyme-linked immunosorbent assay: prognostic and diagnostic potentials. Urology. 75(2): 257-61.

NCBI and Uniprot Product Information

• NCBI GI #: 169636420
• NCBI GeneID: 768
• NCBI Accession #: NP_001207.2
• NCBI GenBank Nucleotide #: NM_001216.2
• UniProt Accession #: Q16790; Q5T4R1
• Molecular Weight: 49698 MW
• NCBI Official Full Name: carbonic anhydrase 9
• NCBI Official Synonym Full Names: carbonic anhydrase 9
• NCBI Official Symbol: CA9
• NCBI Official Synonym Symbols: MN; CAIX
• NCBI Protein Information: carbonic anhydrase 9
• UniProt Protein Name: Carbonic anhydrase 9
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA9
• UniProt Synonym Gene Names: G250; MN; CA-IX; CAIX; RCC-associated antigen G250

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]

Uniprot Description

Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.