Rabbit Actinin-alpha3 Polyclonal Antibody | anti-E25 antibody

Actinin-alpha3 (E25) Polyclonal Antibody

Cat. Num. AAA3002287

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Actinin-alpha3; Polyclonal Antibody; Actinin-alpha3 (E25) Polyclonal Antibody; Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; ACTN3; alpha-Actinin3; Actinin alpha3; Actininalpha3; alphaActinin-3; alpha-actinin-3; anti-E25 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Actinin-alpha3 (E25) polyclonal antibody detects endogenous levels of actinin-alpha3 protein, this antibody does not cross-react with other alpha-actinin isoforms.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 944

• Applicable Applications for anti-E25 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to the N-terminus of Human Actinin-alpha3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Actinin-alpha3 (E25) polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Mouse muscle tissue lysateLane3:Rat muscel tissue lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Actinin-alpha3 (E25) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Actinin-alpha3 (E25) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Actinin-alpha3 (E25) pAb in paraffin-embedded human lung carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Actinin-alpha3 (E25) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Western Blot (WB)

(Western Blot (WB) analysis of Actinin-alpha3 (E25) pAb at 1:500 dilutionLane1:MCF-7 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:BV2 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug))

Related Product Information for anti-E25 antibody

The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and alpha-actinins. There are four tissue-specific alpha-actinins, namely alpha-actinin-1, alpha-actinin-2, alpha-actinin-3 and alpha-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each alpha-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding alpha-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure.

NCBI and Uniprot Product Information

• NCBI GI #: 385648244
• NCBI Accession #: NP_001245300.1
• NCBI GenBank Nucleotide #: NP_001245300.1
• UniProt Accession #: Q08043
• Molecular Weight: ~ 103kDa
• NCBI Official Full Name: alpha-actinin-3 isoform 2
• UniProt Protein Name: Alpha-actinin-3
• UniProt Gene Name: ACTN3
• UniProt Entry Name: ACTN3_HUMAN

Uniprot Description

ACTN3: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Belongs to the alpha-actinin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: focal adhesion; actin filament; pseudopodium; cytosol

Molecular Function: integrin binding; protein binding; protein homodimerization activity; structural constituent of muscle; calcium ion binding; actin binding

Biological Process: regulation of apoptosis; focal adhesion formation; muscle filament sliding