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SDS-PAGE

Carbonic Anhydrase XIII / CA13 Recombinant Protein | CA13 recombinant protein

Recombinant Human Carbonic Anhydrase XIII / CA13 Protein (His tag)

Gene Names
CA13; CAXIII
Purity
> 96 % as determined by SDS-PAGE
Synonyms
Carbonic Anhydrase XIII / CA13; Recombinant Human Carbonic Anhydrase XIII / CA13 Protein (His tag); CAXIII; FLJ37995; MGC59868; CA13 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 96 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 20mM Tris, 0.5M NaCl, pH 8.0
Sequence Length
262
Application Notes
The recombinant human CA13 consisting of 268 amino acids and has a calculated molecular mass of 30.3 kDa. It migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions.
Predicted N Terminal
Met 1
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

SDS-PAGE
Related Product Information for CA13 recombinant protein
Background: The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. The CAXIII is a member of the CA family, which owns a globular molecule with high structural similarity to cytosolic isozymes, CAI, II, and III. Recombinant mouse CAXIII showed catalytic activity similar to those of mitochondrial CAV and cytosolic CAI. In human tissues, CAXIII expression was identified in the thymus, small intestine, spleen, prostate, ovary, colon, and testis. In mouse, positive tissues included the spleen, lung, kidney, heart, brain, skeletal muscle, and testis. In conclusion, the predicted amino acid sequence, structural model, distribution, and activity data suggest that CAXIII represents a novel enzyme, which may play important physiological roles in several organs.

Description: A DNA sequence encoding the human CA13 (NP_940986.1) (Met 1-His 262) was expressed, with a polyhistide tag at the C-terminus.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,443 Da
NCBI Official Full Name
carbonic anhydrase 13
NCBI Official Synonym Full Names
carbonic anhydrase 13
NCBI Official Symbol
CA13
NCBI Official Synonym Symbols
CAXIII
NCBI Protein Information
carbonic anhydrase 13
UniProt Protein Name
Carbonic anhydrase 13
Protein Family
UniProt Gene Name
CA13
UniProt Synonym Gene Names
CA-XIII

NCBI Description

Carbonic anhydrases (CAs) are a family of zinc metalloenzymes. For background information on the CA family, see MIM 114800.[supplied by OMIM, Mar 2008]

Uniprot Description

CA13: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.

Protein type: EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase

Chromosomal Location of Human Ortholog: 8q21.2

Cellular Component: cytosol

Molecular Function: carbonate dehydratase activity

Biological Process: bicarbonate transport

Research Articles on CA13

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Product Notes

The CA13 ca13 (Catalog #AAA2546247) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The recombinant human CA13 consisting of 268 amino acids and has a calculated molecular mass of 30.3 kDa. It migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the CA13 ca13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase XIII / CA13, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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