SDS-Page
CA13 active protein
CA13 Protein, Human, Recombinant (His Tag)
Gene Names
CA13; CAXIII
Purity
>96% as determined by SDS-PAGE
Synonyms
CA13; CA13 Protein; Human; Recombinant (His Tag); Human Carbonic Anhydrase XIII/CA13 Protein (His Tag); CAXIII Protein; FLJ37995 Protein; MGC59868 Protein; carbonic anhydrase XIII; CA13 active protein
Host
E Coli
Purity/Purification
>96% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 20mM Tris, 0.5M NaCl, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-His262
Species
Human
Activity
Measured by its esterase activity. The specific activity is >7pmoles/min/ug.
Predicted N Terminal
Met 1
Tag
C-His
Protein Construction
A DNA sequence encoding the human CA13 (NP_940986.1)(Met 1-His 262) was expressed, with a polyhistide tag at the C-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for CA13 active protein
Background: The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. The CAXIII is a member of the CA family, which owns a globular molecule with high structural similarity to cytosolic isozymes, CAI, II, and III. Recombinant mouse CAXIII showed catalytic activity similar to those of mitochondrial CAV and cytosolic CAI. In human tissues, CAXIII expression was identified in the thymus, small intestine, spleen, prostate, ovary, colon, and testis. In mouse, positive tissues included the spleen, lung, kidney, heart, brain, skeletal muscle, and testis. In conclusion, the predicted amino acid sequence, structural model, distribution, and activity data suggest that CAXIII represents a novel enzyme, which may play important physiological roles in several organs.
References
Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1): 1-20.Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,443 Da
NCBI Official Full Name
carbonic anhydrase 13
NCBI Official Synonym Full Names
carbonic anhydrase XIII
NCBI Official Symbol
CA13
NCBI Official Synonym Symbols
CAXIII
NCBI Protein Information
carbonic anhydrase 13; CA-XIII; carbonate dehydratase XIII
UniProt Protein Name
Carbonic anhydrase 13
Protein Family
UniProt Gene Name
CA13
UniProt Synonym Gene Names
CA-XIII
UniProt Entry Name
CAH13_HUMAN
NCBI Description
Carbonic anhydrases (CAs) are a family of zinc metalloenzymes. For background information on the CA family, see MIM 114800.[supplied by OMIM, Mar 2008]
Uniprot Description
CA13: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.
Protein type: Energy Metabolism - nitrogen; EC 4.2.1.1; Lyase
Chromosomal Location of Human Ortholog: 8q21.2
Cellular Component: cytosol; myelin sheath
Molecular Function: carbonate dehydratase activity; zinc ion binding
Biological Process: bicarbonate transport; one-carbon compound metabolic process
Research Articles on CA13
Similar Products
Product Notes
The CA13 ca13 (Catalog #AAA8120285) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-His26 2. It is sometimes possible for the material contained within the vial of "CA13, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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