TYRP1 recombinant protein

TYRP1, 25-477aa, Human, His-tag, Baculovirus

Cat. Num. AAA206442

• Gene Names: TYRP1; TRP; CAS2; CATB; GP75; OCA3; TRP1; TYRP; b-PROTEIN
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: TYRP1; 25-477aa; Human; His-tag; Baculovirus; 5; 6-dihydroxyindole-2-carboxylic acid oxidase; b-PROTEIN; CAS2; CATB; GP75; OCA3; TRP; TRP1; TYRP; TYRP1 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADLQFPRQCA TVEALRSGMC CPDLSPVSGP GTDRCGSSSG RGRCEAVTAD SRPHSPQYPH DGRDDREVWP LRFFNRTCHC NGNFSGHNCG TCRPGWRGAA CDQRVLIVRR NLLDLSKEEK NHFVRALDMA KRTTHPLFVI ATRRSEEILG PDGNTPQFEN ISIYNYFVWT HYYSVKKTFL GVGQESFGEV DFSHEGPAFL TWHRYHLLRL EKDMQEMLQE PSFSLPYWNF ATGKNVCDIC TDDLMGSRSN FDSTLISPNS VFSQWRVVCD SLEDYDTLGT LCNSTEDGPI RRNPAGNVAR PMVQRLPEPQ DVAQCLEVGL FDTPPFYSNS TNSFRNTVEG YSDPTGKYDP AVRSLHNLAH LFLNGTGGQT HLSPNDPIFV LLHTFTDAVF DEWLRRYNAD ISTFPLENAP IGHNRQYNMV PFWPPVTNTE MFVTAPDNLG YTYEIQWPSR EFSVPEHHHH HH
• Sequence Length: 537

• Applicable Applications for TYRP1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for TYRP1 recombinant protein

TYRP1, also known as 5, 6-dihydroxyindole-2-carboxylic acid oxidase, is a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway. It is a melanocyte-specific gene product involved in eumelanin synthesis. It is involved in the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. It is regulated by the microphthalmia-associated transcription factor (MITF). Recombinant human TYRP1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for TYRP1 recombinant protein

Cancer

References

Sarangarajan R., et al. (2001) Pigment Cell Res. 14:437-444.; Chalupa A., et al. (2015) J Invest Dermatol. 135:202-211.

NCBI and Uniprot Product Information

• NCBI GI #: 4507757
• NCBI GeneID: 7306
• NCBI Accession #: NP_000541
• NCBI GenBank Nucleotide #: NM_000550.2
• UniProt Accession #: P17643; P78468; P78469; Q13721; Q15679
• Molecular Weight: 52.5kDa (462aa) 50-70kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: 5,6-dihydroxyindole-2-carboxylic acid oxidase
• NCBI Official Synonym Full Names: tyrosinase related protein 1
• NCBI Official Symbol: TYRP1
• NCBI Official Synonym Symbols: TRP; CAS2; CATB; GP75; OCA3; TRP1; TYRP; b-PROTEIN
• NCBI Protein Information: 5,6-dihydroxyindole-2-carboxylic acid oxidase
• UniProt Protein Name: 5,6-dihydroxyindole-2-carboxylic acid oxidase
• Protein Family: 5,6-dihydroxyindole-2-carboxylic acid oxidase
• UniProt Gene Name: TYRP1
• UniProt Synonym Gene Names: TRP-1; TRP1

NCBI Description

This gene encodes a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway. Defects in this gene are the cause of rufous oculocutaneous albinism and oculocutaneous albinism type III. [provided by RefSeq, Mar 2009]

Uniprot Description

Catalyzes the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid in the presence of bound Cu2+ ions (PubMed:28661582). May regulate or influence the type of melanin synthesized (PubMed:22556244, PubMed:16704458). Also to a lower extent, capable of hydroxylating tyrosine and producing melanin ().