Western Blot (WB)
(Anti-CA13 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:29 kDaObserved band size:33 kDa)
Rabbit anti-Human CA13 Polyclonal Antibody | anti-CA13 antibody
Anti-CA13 Antibody, Rabbit Polyclonal
Gene Names
CA13; CAXIII
Reactivity
Human
Applications
Western Blot, ELISA, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
CA13; Polyclonal Antibody; Anti-CA13 Antibody; Rabbit Polyclonal; Carbonic Anhydrase XIII/CA13 Antibody; Rabbit PAb; Antigen Affinity Purified; carbonic anhydrase XIII; Anti-CAXIII Antibody; Anti-FLJ37995 Antibody; Anti-MGC59868 Antibody; anti-CA13 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Carbonic Anhydrase XIII/CA13
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CA13 antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
IP: 4-6uL/mg of lysate
ELISA: 1:5000-1:10,000
IP: 4-6uL/mg of lysate
Immunogen
Recombinant Human Carbonic Anhydrase XIII/CA13 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase XIII/CA13 (rh Carbonic Anhydrase XIII/CA13). Carbonic Anhydrase XIII/CA13 specific IgG was purified by Human Carbonic Anhydrase XIII/CA13 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-CA13 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:29 kDaObserved band size:33 kDa)
Western Blot (WB)
(Anti-CA13 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:29 kDaObserved band size:33 kDa)
Immunoprecipitation (IP)
(CA13 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate2 uL anti-CA13 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CA13 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size: 29 kDa)
Immunoprecipitation (IP)
(CA13 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate2 uL anti-CA13 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CA13 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size: 29 kDa)
Related Product Information for anti-CA13 antibody
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. The CAXIII is a member of the CA family, which owns a globular molecule with high structural similarity to cytosolic isozymes, CAI, II, and III. Recombinant mouse CAXIII showed catalytic activity similar to those of mitochondrial CAV and cytosolic CAI. In human tissues, CAXIII expression was identified in the thymus, small intestine, spleen, prostate, ovary, colon, and testis. In mouse, positive tissues included the spleen, lung, kidney, heart, brain, skeletal muscle, and testis. In conclusion, the predicted amino acid sequence, structural model, distribution, and activity data suggest that CAXIII represents a novel enzyme, which may play important physiological roles in several organs.
References
Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,443 Da
NCBI Official Full Name
carbonic anhydrase 13
NCBI Official Synonym Full Names
carbonic anhydrase XIII
NCBI Official Symbol
CA13
NCBI Official Synonym Symbols
CAXIII
NCBI Protein Information
carbonic anhydrase 13; CA-XIII; carbonate dehydratase XIII
UniProt Protein Name
Carbonic anhydrase 13
Protein Family
UniProt Gene Name
CA13
UniProt Synonym Gene Names
CA-XIII
UniProt Entry Name
CAH13_HUMAN
NCBI Description
Carbonic anhydrases (CAs) are a family of zinc metalloenzymes. For background information on the CA family, see MIM 114800.[supplied by OMIM, Mar 2008]
Uniprot Description
CA13: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.
Protein type: Energy Metabolism - nitrogen; EC 4.2.1.1; Lyase
Chromosomal Location of Human Ortholog: 8q21.2
Cellular Component: cytosol; myelin sheath
Molecular Function: carbonate dehydratase activity; zinc ion binding
Biological Process: bicarbonate transport; one-carbon compound metabolic process
Research Articles on CA13
Similar Products
Product Notes
The CA13 ca13 (Catalog #AAA8102885) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CA13 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CA13 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IP: 4-6uL/mg of lysate. Researchers should empirically determine the suitability of the CA13 ca13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CA13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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