Rabbit anti-Mouse Ddr2 Polyclonal Antibody | anti-Ddr2 antibody

Ddr2, CT (Ddr2, Ntrkr3, Tkt, Tyro10, Discoidin domain-containing receptor 2, CD167 antigen-like family member B, Neurotrophic tyrosine kinase, receptor-related 3, Receptor protein-tyrosine kinase TKT, Tyrosine-protein kinase TYRO10, CD167b) (MaxLight 650)

Cat. Num. AAA6291329

• Gene Names: Ddr2; Ntrkr3; tyro10; AW495251
• Reactivity: Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Ddr2; Polyclonal Antibody; CT (Ddr2; Ntrkr3; Tkt; Tyro10; Discoidin domain-containing receptor 2; CD167 antigen-like family member B; Neurotrophic tyrosine kinase; receptor-related 3; Receptor protein-tyrosine kinase TKT; Tyrosine-protein kinase TYRO10; CD167b) (MaxLight 650); CD167b; anti-Ddr2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Ddr2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-Ddr2 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 816-843 in the C-terminal region of mouse Ddr2.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse bladder tissue lysates (35ug/lane) usingMBS644034. This demonstrates that MBS644034 detected the Ddr2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue using MBS644034 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS644034 for immunohistochemistry.)

Product Categories/Family for anti-Ddr2 antibody

Antibodies; Protein Kinases

References

Xu, L., et al. Arthritis Rheum. 62(9):2736-2744(2010). Sivakumar, L., et al. Biomaterials 31(18):4802-4808(2010). Flynn, L.A., et al. J. Mol. Biol. 395(3):533-543(2010). Kano, K., et al. Mol. Reprod. Dev. 77(1):29-37(2010). Matsumura, H., et al. Physiol. Genomics 39(2):120-129(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 158508514
• NCBI GeneID: 18214
• NCBI Accession #: NP_072075.2
• NCBI GenBank Nucleotide #: NM_022563.2
• UniProt Accession #: Q62371; B2RSD7
• Molecular Weight: 96,482 Da
• NCBI Official Full Name: discoidin domain-containing receptor 2
• NCBI Official Synonym Full Names: discoidin domain receptor family, member 2
• NCBI Official Symbol: Ddr2
• NCBI Official Synonym Symbols: Ntrkr3; tyro10; AW495251
• NCBI Protein Information: discoidin domain-containing receptor 2
• UniProt Protein Name: Discoidin domain-containing receptor 2
• Protein Family: Discoidin domain-containing receptor
• UniProt Gene Name: Ddr2
• UniProt Synonym Gene Names: Ntrkr3; Tkt; Tyro10; Discoidin domain receptor 2
• UniProt Entry Name: DDR2_MOUSE

Uniprot Description

DDR2: This tyrosine kinase receptor for fibrillar collagen mediates fibroblast migration and proliferation. Contributes to cutaneous wound healing. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Protein type: Protein kinase, TK; Kinase, protein; Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; DDR family

Cellular Component: focal adhesion; membrane; integral to plasma membrane; apical plasma membrane; integral to membrane; plasma membrane

Molecular Function: transferase activity; collagen binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: ossification; collagen fibril organization; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation; positive regulation of osteoblast differentiation; positive regulation of fibroblast proliferation; biomineral formation; positive regulation of protein kinase activity; positive regulation of cell proliferation; positive regulation of transcription factor activity; phosphorylation; regulation of bone mineralization; transmembrane receptor protein tyrosine kinase signaling pathway